氧化羧甲基纤维素钠改性胶原膜的制备及表征

doi:10.19677/j.issn.1004-7964.2020.04.002

皮革科学与工程 ›› 2020, Vol. 30 ›› Issue (4): 7-12.doi: 10.19677/j.issn.1004-7964.2020.04.002

氧化羧甲基纤维素钠改性胶原膜的制备及表征

田振华^1,2,*, 王颖¹

1.陕西科技大学轻工科学与工程学院,陕西西安 710021;
2.轻化工程国家级实验教学示范中心（陕西科技大学）,陕西西安 710021

收稿日期:2020-01-03 出版日期:2020-08-28 上线日期:2020-07-26
通讯作者: *
作者简介:田振华（1989-）,女,博士,讲师,研究方向：胶原基材料制备及构效关系研究,电话：13572965526,E-mail:tian_amb@163.com。
基金资助:
国家自然科学基金项目(21706151); 陕西省自然科学基础研究计划项目(2019JQ-027); 轻化工程国家级实验教学示范中心（陕西科技大学）开放课题(2018QGSJ02-16)

Preparation and Characterization of Collagen Membranes Modified by Oxidized Sodium Carboxymethylcellulose

TIAN Zhenhua^1,2,*, WANG Ying¹

1. College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;
2. National Experimental Teaching Demonstration Center of Light Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China

Received:2020-01-03 Online:2020-08-28 Published:2020-07-26

摘要/Abstract

摘要： 采用不同用量的交联剂—氧化羧甲基纤维素钠（OCMC）对胶原[m(OCMC):m(胶原) = 0~10:1]溶液进行改性,制备均一的改性胶原膜,并对其物理化学性质进行表征。红外光谱显示,改性胶原与纯胶原的结构基本一致,具有完整的三股螺旋结构,但胶原分子间氢键作用被削弱。通过对胶原膜的热稳定性、溶胀率和接触角分析发现,其理化性质的变化与交联剂的用量密切相关。当m(OCMC):m(胶原)≤5:1时,随着OCMC用量的增大,胶原氨基与OCMC醛基之间交联作用显著上升,使得胶原膜的热变性温度和接触角分别由86.6 ℃和87.0°大幅上升至98.4 ℃和105.0°,溶胀率由880%下降到448%,胶原氨基被大量消耗。因此,当m(OCMC):m(胶原) > 5:1时,即使交联剂用量成倍增加,胶原膜的热变性温度和接触角仅分别上升2.5 ℃和3.1°,溶胀率仅下降62%。

关键词: 胶原, 氧化羧甲基纤维素钠, 交联改性, 理化性质

Abstract: Collagen in aqueous solution was modified with different dosages of oxidized sodium carboxymethylcellulose (OCMC) [m(OCMC):m(collagen) = 0~10:1] as a cross-linking agent, and the corresponding modified collagen membranes were then prepared and characterized by various techniques. Results of Fourier transform infrared spectroscopic analysis indicated that the triple-helical structure of collagen was not demolished, but the hydrogen bonding among collagen molecules was weakened due to the introduction of cross-linking linkages. According to the results of differential scanning calorimetry, swelling and water contact angle tests, it was found that the collagen membrane possessed strikingly different physicochemical properties depending on the dosage of OCMC. When the ratio of OCMC/collagen is less than 5:1, the cross-linking between amino groups of collagen and aldehyde groups of OCMC increased significantly with the increase of OCMC dosage. Especially, at the 5:1 ratio of OCMC/collagen, both thermal denaturation temperature (T_d) and water contact angle (WCA) of collagen membrane were improved significantly from the original 86.6 ℃ and 87.0° to 98.4 ℃ and 105.0°, respectively, and the swelling ratio of collagen membrane was dramatically reduced from the original 880% to 448%. However, when the ratio of OCMC/collagen is more than 5:1, the increase of OCMC dosage has no remarkable effect on the physicochemical properties of collagen membrane. For example, as the OCMC/collagen ratio was multiplied from 5:1 to 10:1, the increased values of Td and WCA were only 2.5 ℃ and 3.1°, respectively, while the decreased value of swelling ratio was only 62%.

Key words: collagen, oxidized sodium carboxymethylcellulose, cross-linking modification, physicochemical property

中图分类号:

TS512

田振华, 王颖. 氧化羧甲基纤维素钠改性胶原膜的制备及表征[J]. 皮革科学与工程, 2020, 30(4): 7-12.

TIAN Zhenhua, WANG Ying. Preparation and Characterization of Collagen Membranes Modified by Oxidized Sodium Carboxymethylcellulose[J]. Leather Science and Engineering, 2020, 30(4): 7-12.

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氧化羧甲基纤维素钠改性胶原膜的制备及表征

Preparation and Characterization of Collagen Membranes Modified by Oxidized Sodium Carboxymethylcellulose

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