不同结构的多酚交联对胶原水凝胶理化性质的影响

doi:10.19677/j.issn.1004-7964.2023.05.001

皮革科学与工程 ›› 2023, Vol. 33 ›› Issue (5): 1-7.doi: 10.19677/j.issn.1004-7964.2023.05.001

• 试验研究 • 下一篇

不同结构的多酚交联对胶原水凝胶理化性质的影响

乐薇^1,2, 张旭³, 秦子波^1,2, 余小月^1,2, 熊善柏^1,2, 胡杨^1,2,4,*

1.华中农业大学食品科学技术学院,湖北武汉 430070;
2.国家大宗淡水鱼加工技术研发分中心,湖北武汉 430070;
3.广东省医疗器械质量监督检验所,广东广州 510663;
4.湖北省生物活性肽技术工程研究中心,湖北荆州 434000

收稿日期:2023-01-09 出版日期:2023-10-01 上线日期:2023-09-20
通讯作者: *胡杨（1987-）,男,博士,副教授,博士生导师,研究方向为水产品加工及副产物高值化利用。E-mail: huyang@mail.hzau.edu.cn。
作者简介:乐薇（1999-）,女,硕士研究生,研究方向为水产品加工及副产物高值化利用。E-mail: Yuewei0202@163.com。
基金资助:
湖北省自然科学基金面上项目（2022CFB162）

Effect of Crosslinking by Polyphenols with Different Structures on the Physicochemical Properties of Collagen Hydrogel

YUE Wei^1,2, ZHANG Xu³, QIN Zibo^1,2, YU Xiaoyue^1,2, XIONG Shanbai^1,2, HU Yang^1,2,4,*

1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. National Bulk Freshwater Fish Processing Technology Research and Development Sub-Center, Wuhan 430070, China;
3. Guangdong Medical Devices Quality Surveillance and Test Institute, Guangzhou 510663, China;
4. Bioactive Peptide Technology Engineering Research Center, Jingzhou 434000, China

Received:2023-01-09 Online:2023-10-01 Published:2023-09-20

摘要/Abstract

摘要： 牛跟腱胶原水凝胶在生物医用材料等领域应用广泛,但理化特性有待提升。选取原花青素（PA）、没食子酸、表没食子儿茶素没食子酸酯3种典型多酚对胶原水凝胶进行交联,通过测定水凝胶持水性、溶胀率、力学性能等,研究多酚结构对水凝胶理化特性的影响。结果表明,3种多酚交联后的水凝胶持水性有所下降,但均在95%以上。随着多酚浓度增加,水凝胶溶胀率均有所降低,力学性能、耐酶解性和动态流变学特性逐渐增强,其中9 mmol/L PA交联的水凝胶理化特性提升显著,硬度达882 g,降解率仅为2.47%,储能模量约在4 000 Pa以上。研究了3种多酚对胶原水凝胶的交联改性效果,可望为牛跟腱的高值化利用提供一定理论和技术支撑。

关键词: 牛跟腱, 胶原, 水凝胶, 多酚, 交联

Abstract: Collagen hydrogel of bovine achilles tendon can be widely used in biomedical materials and other fields, but its physical and chemical properties need to be improved. In this study, three typical polyphenols, proanthocyanidins(PA), gallic acid, and epigallocatechin gallate, were selected to crosslink collagen hydrogels, and the water holding capacity, swelling rates, mechanical properties and rheological properties of the obtained cross-linked hydrogels as well as the micro-morphology of assembled collagen molecules were measured. The results showed that the water holding capacity of collagen hydrogel decreased after being crosslinked by three polyphenols, but all of them were above 95%. With the increase of polyphenol concentration, the swelling rate of hydrogel decreased, and the mechanical properties, enzymatic hydrolysis resistance and dynamic rheological properties were gradually enhanced, among which the physicochemical properties of collagen hydrogel crosslinked by 9 mmol/L PA were significantly improved. The hardness reached to 882 g; the degradation rate was only 2.47%; the energy storage modulus was greater than 4 000 Pa. This work is expected to provide certain theoretical and technical support for the high-value utilization of bovine achilles tendon.

Key words: bovine achilles tendon, collagen, hydrogel, polyphenols, crosslinking

中图分类号:

TS512

乐薇, 张旭, 秦子波, 余小月, 熊善柏, 胡杨. 不同结构的多酚交联对胶原水凝胶理化性质的影响[J]. 皮革科学与工程, 2023, 33(5): 1-7.

YUE Wei, ZHANG Xu, QIN Zibo, YU Xiaoyue, XIONG Shanbai, HU Yang. Effect of Crosslinking by Polyphenols with Different Structures on the Physicochemical Properties of Collagen Hydrogel[J]. Leather Science and Engineering, 2023, 33(5): 1-7.

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不同结构的多酚交联对胶原水凝胶理化性质的影响

Effect of Crosslinking by Polyphenols with Different Structures on the Physicochemical Properties of Collagen Hydrogel

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