漆酶-原儿茶醛交联鱼皮胶原蛋白水凝胶的研究

doi:10.19677/j.issn.1004-7964.2024.02.001

皮革科学与工程 ›› 2024, Vol. 34 ›› Issue (2): 1-6.doi: 10.19677/j.issn.1004-7964.2024.02.001

• 试验研究 • 下一篇

漆酶-原儿茶醛交联鱼皮胶原蛋白水凝胶的研究

杨长凯^1,2, 李国英^1,2,*

1.四川大学皮革化学与工程教育部重点实验室,四川成都 610065;
2.四川大学制革清洁技术国家工程实验室,四川成都 610065

收稿日期:2023-09-11 修回日期:2023-11-20 接受日期:2023-11-22 出版日期:2024-04-01 上线日期:2024-03-07
通讯作者: *李国英（1967-）,女,教授,博士生导师,研究方向为胶原化学及其生物学利用。E-mail：liguoyings@163.com。
作者简介:杨长凯（1993-）,男,博士研究生,研究方向为生物质化学与工程。E-mail：1120417956@qq.com。
基金资助:
国家自然科学基金资助项目（22078206）

Study on the Collagen Hydrogel of Fish Skin via Laccase- Protocatechuic Aldehyde Cross-linking

YANG Changkai^1,2, LI Guoying^1,2,*

1. Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China;
2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China

Received:2023-09-11 Revised:2023-11-20 Accepted:2023-11-22 Published:2024-04-01 Online:2024-03-07

摘要/Abstract

摘要： 与哺乳动物蛋白相比,鱼皮胶原蛋白未受到克雅氏病和牛海绵状脑病流行的限制,具有较大的应用潜力,然而较差的力学性能和耐降解性质限制了鱼皮胶原的应用。论文探究了漆酶（LAC）和原儿茶醛（PAL）交联制备胶原蛋白水凝胶的交联机理,凝胶微观结构,力学性能和耐降解性质。结果表明,漆酶能够交联胶原成胶。同时,原儿茶醛的引入增强了漆酶交联胶原的作用,提升了胶原蛋白水凝胶的结构稳定性和耐降解性质。LAC-PAL交联胶原是一种构建胶原蛋白水凝胶的有效策略。

关键词: 水凝胶, 鱼皮, 胶原, 漆酶, 原儿茶醛

Abstract: Fish skin collagen has greater application potential than mammalian proteins because it is not limited by the prevalence of Creutzfeldt-Jakob disease and bovine spongiform encephalopathy. However, it is restricted by its poor mechanical properties and poor resistance to degradation. This work investigated the cross-linking mechanism, gel microstructure, mechanical properties and degradation resistance of collagen hydrogels prepared by the cross-linking of laccase (LAC) with protocatechuic aldehyde (PAL). Results showed that LAC could cross-link collagen to form a hydrogel. Meanwhile, the introduction of PAL enhanced the role of LAC in cross-linking collagen and improved the structural stability and degradation resistance of the resulting collagen hydrogels. The collagen cross-linked via LAC-PAL is an effective strategy for constructing collagen hydrogels.

Key words: hydrogel, fish skin, collagen, laccase, protocatechuic aldehyde

中图分类号:

TS512

杨长凯, 李国英. 漆酶-原儿茶醛交联鱼皮胶原蛋白水凝胶的研究[J]. 皮革科学与工程, 2024, 34(2): 1-6.

YANG Changkai, LI Guoying. Study on the Collagen Hydrogel of Fish Skin via Laccase- Protocatechuic Aldehyde Cross-linking[J]. Leather Science and Engineering, 2024, 34(2): 1-6.

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漆酶-原儿茶醛交联鱼皮胶原蛋白水凝胶的研究

Study on the Collagen Hydrogel of Fish Skin via Laccase- Protocatechuic Aldehyde Cross-linking

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