皮革科学与工程 ›› 2019, Vol. 29 ›› Issue (05): 16-22.doi: 10.19677/j.issn.1004-7964.2019.05.003

• 论文 • 上一篇    下一篇

胎牛皮源高层级胶原聚集体的制备与表征

  

  1. 四川大学皮革化学与工程教育部重点实验室;四川大学生物医学工程技术研究中心;
  • 出版日期:2019-10-12 上线日期:2019-10-12
  • 基金资助:
    国家自然科学基金(编号:514730001)

The Controllable Preparation of Aggregated Collagen based on Traceable Fetal Bovine Skin

  1. 1. The Key Laboratory of Leather and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China; 2. Biomedical Engineering Technology Research Centre，Sichuan University, Chengdu 61006, China
  • Online:2019-10-12 Published:2019-10-12

PDF

35

摘要： 以实验室自制胎牛皮脱细胞真皮基质(FB-ADM)为原料,采用自主设计的可控降解技术制备了胶原(col)和超分子聚集态胶原结构体(高层级胶原聚集体,AG-col),流延成膜后,通过AFM、FT-IR、CD、DSC、TG、抗张强度检测、耐酶降解性能检测等手段对col和AG-col的性能结构进行表征,结果表明:二者红外特征吸收峰明显,三股螺旋结构完整,但AG-col的结构层次更高级,交联结构更加稳定,热稳定性(耐热破坏性和抗热分解能力)更好。此外,AG-col的强度、柔韧性和耐酶降解性能较col也更加优越。

关键词: 胶原, 胶原聚集体, 胎牛皮

Abstract: In this study, by self-designed controllable-degradation method, collagen(col) and aggregated collagen（AG-col) were prepared from fetal bovine acellular dermal matrix (FB -ADM). Atomic force microscope (AFM), Fourier transform infrared spectroscopy(FTIR),circular dichroism (CD),differential scanning calorimetry(DSC), thermogravimetric analysis (TG), mechanical tests and collagenase degradation test were conducted to characterize col and AG -col. Results showed that both of them owned obvious characteristic peaks in FTIR spectra, indirectly demonstrating the exist of their characteristic triple helix structure. However, AG-col is more advanced than col, with better thermal stability. Furthermore, the mechanical property and resistance to enzymatic degradation of AG-col were also superior to col.

Key words: collagen, aggregated collagen, fetal bovine skin

中图分类号: 

网站版权©2019《皮革科学与工程》杂志社

编辑部地址：成都市一环路南一段24号四川大学皮革楼《皮革科学与工程》杂志社（610065）

本系统由北京玛格泰克科技发展有限公司设计开发