电解质诱导胶原在云母基底上的类外延附生有序聚集

doi:10.19677/j.issn.1004-7964.2021.01.003

皮革科学与工程 ›› 2021, Vol. 31 ›› Issue (1): 14-19.doi: 10.19677/j.issn.1004-7964.2021.01.003

电解质诱导胶原在云母基底上的类外延附生有序聚集

魏香奕^1,2, 代娟^1,2, 袁亚^1,2, 孙警辉^1,2, 李学理^1,2, 刘玲玲^1,2,*

1.成都医学院四川省动物源性食品兽药残留防控技术工程实验室,四川成都 610500;
2.成都医学院检验医学院,四川成都 610500

收稿日期:2020-05-10 上线日期:2021-05-28
通讯作者: *E- mail:liulingling2012@163.com
作者简介:魏香奕（1983-）,女,博士,讲师。研究方向：生物质资源利用,E-mail: weixiangyi2017@qq.com。
基金资助:
四川省教育厅科研重点项目(16ZA0295)

Electrolyte-induced Quasi-epitaxial Ordered Assembly of Collagen on Mica Surface

WEI Xiangyi^1,2, DAI Juan^1,2, YUAN Ya^1,2, SUN Jinghui^1,2, LI Xueli^1,2, LIU Lingling^1,2,*

1. Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin Food, Chengdu Medical College, Chengdu 610500, China;
2. School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China

Received:2020-05-10 Online:2021-05-28

摘要/Abstract

摘要： 使用AFM研究了电解质诱导的胶原在云母基底上的聚集行为。实验结果表明胶原的聚集行为受到电解质类型和浓度的影响。某些电解质,例如KCl、K₂SO₄、(NH₄)₂SO₄等,能够使胶原表面电荷由正转负,进而诱导胶原在云母基底上的类外延附生现象,胶原聚集结构走向与云母基底的晶格方向一致。胶原的外延附生现象,可能是由胶原和云母表面电荷带的相互作用产生的。研究结果可为生物大分子的有序聚集提供参考。

关键词: 胶原, 类外延附生, 云母, 电解质, 有序聚集

Abstract: Electrolyte-induced assembly behavior of collagen on mica surface was investigated by atomic force microscope (AFM). It was found that the assembly behavior of collagen depended on the electrolyte species and their concentrations. Some electrolytes, KCl, K₂SO₄, (NH₄)₂SO₄, etc., could lead to a conversion of positive zeta potential of collagen solution into negative one, and further induce the quasi-epitaxial growth of collagen on mica substrate. The orientation of the collagen assembly structure was consistent with the crystallographic direction of mica substrate. The quasi-epitaxial assembly of collagen may be caused by the interaction between collagen and the charged strips on mica surface. The resent research results could provide the beneficial reference for the hierarchical and ordered assembly of biomacromolecules.

Key words: collagen, quasi-epitaxial epiphyte, mica, electrolyte, ordered assembly

中图分类号:

TS512

魏香奕, 代娟, 袁亚, 孙警辉, 李学理, 刘玲玲. 电解质诱导胶原在云母基底上的类外延附生有序聚集[J]. 皮革科学与工程, 2021, 31(1): 14-19.

WEI Xiangyi, DAI Juan, YUAN Ya, SUN Jinghui, LI Xueli, LIU Lingling. Electrolyte-induced Quasi-epitaxial Ordered Assembly of Collagen on Mica Surface[J]. Leather Science and Engineering, 2021, 31(1): 14-19.

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电解质诱导胶原在云母基底上的类外延附生有序聚集

Electrolyte-induced Quasi-epitaxial Ordered Assembly of Collagen on Mica Surface

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