关键词: 不确定度, UHPLC-MS/MS, 壬基酚, 鞋类, 外标法

Abstract: The residues of alkylphenol and o-phenylphenol in footwear were determined by ultra high performance liquid chromatography tandem-mass spectrometry (UHPLC-MS/MS), and the uncertainty analysis model was established by using nonylphenol as the representative. Through the evaluation and synthesis of measurement uncertainty sources such as sample weighing, constant volume, repeatability determination, standard material, working curve drawing and fitting, instrument calibration and so on, the results showed that when the content of nonylphenol in the sample was 5.07 mg/kg, the expanded was 0.81 mg/kg (k=2), and the main source of uncertainty was instrument calibration and standard working curve drawing.

Key words: uncertainty, UHPLC-MS/MS, nonylphenol (NP), footwear, external standard method