关键词: 脱铬皮屑, 降解, 发酵, 菌株筛选

Abstract: In order to obtain an original strain, which can not only efficiently degrade the dechromed wet blue shavings but also be used as agricultural probiotics, the initial screening and re-screening experiments were carried out by using the dechromed wet blue shavings as the main nutrient source for microbial growth. A high-efficiency collagen-degrading strain named P-4 was selected from 6 strains. This bacterial strain was then used to degrade the dechromed wet blue shavings by fermentation, and the fermentation broth which was rich in free amino acids and short peptides was obtained. Results of the fermentation experiments showed that, compared with LB medium, basic medium was the optimal fermentation medium for the fermentation of the dechromed wet blue shavings with strain P-4. Through the 16S rDNA identification, strain P-4 was preliminarily identified as Bacillus licheniformis, which belongs to probiotics commonly used in agriculture.

Key words: dechromed wet blue shavings, degradation, fermentation, strain screening