环氧透明质酸改性脱细胞猪真皮基质的亲水保湿性研究

doi:10.19677/j.issn.1004-7964.2022.03.004

皮革科学与工程 ›› 2022, Vol. 32 ›› Issue (3): 18-23.doi: 10.19677/j.issn.1004-7964.2022.03.004

环氧透明质酸改性脱细胞猪真皮基质的亲水保湿性研究

丁壮¹, 黄轩涛¹, 但年华^1,2,*

1.四川大学皮革化学与工程教育部重点实验室,四川成都 610065;
2.四川大学制革清洁技术国家工程实验室,四川成都 610065

收稿日期:2021-10-15 出版日期:2022-06-28 上线日期:2022-05-16
通讯作者: ^*但年华（1975-）,男,副教授,E-mail:dannianhua@scu.edu.cn,主要从事生物医用材料研究。
作者简介:丁壮（1994-）,男,硕士生。
基金资助:
皮革化学与工程教育部重点实验室（四川大学）开发课题基金资助项目（SCU2021D005）; 中央高校基本科研业务费专项资金（专职博士后）资助项目（2082604E4156）

Study on the Hydrophilicity and Moisturizing of Acellular Porcine Dermal Matrix Crosslinked by Epoxidized Hyaluronic Acid

DING Zhuang¹, HUANG Xuantao¹, DAN Nianhua^1,2,*

1. Key Laboratory of Leather Chemistry and Engineer of Ministry of Education, Sichuan University, Chengdu 610065,China;
2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065,China

Received:2021-10-15 Online:2022-06-28 Published:2022-05-16

摘要/Abstract

摘要： 湿性愈合理论证明了创面修复过程中,保持伤口湿润环境至关重要。为了改善脱细胞猪真皮基质（pADM）敷料的亲水保湿性能,采用环氧化透明质酸（EHA）与pADM交联改性,将高亲水保湿的透明质酸（HA）分子引入到pADM中,制备得到环氧透明质酸改性脱细胞猪真皮基质（EHA-pADM）。采用傅里叶红外光谱仪、原子力显微镜、收缩温度测定仪、差示扫描量热仪等对不同用量EHA改性得到的EHA-pADM进行分析。结果表明：EHA-pADM热稳定性随着EHA用量的增加而升高,产生了交联效应,且EHA-pADM仍然保持着pADM原有的二级结构、三股螺旋结构、横纹结构等微观结构特征。EHA-pADM的表面接触角随着EHA用量的增加而降低,其吸湿率和保水率与EHA用量基本正相关。吸湿动力学分析显示,EHA-pADM对水的吸附量随着EHA用量的增加而提高,吸附特征符合二级动力学方程,吸附过程属于多分子层吸附。可见,HA分子的引入,提高了pADM的表面亲水性能,增加了其保水“锁水”能力。当EHA-pADM用作敷料时,有望成为一种新型的亲水保湿型医用敷料。

关键词: 环氧透明质酸, 脱细胞猪真皮基质, 交联, 亲水性, 保湿性, 生物敷料

Abstract: The theory of moist healing proves that it is essential to maintain a moist environment during wound repair. In order to improve the hydrophilicity and moisturizing of the acellular porcine dermal matrix (pADM) dressing, epoxidized hyaluronic acid (EHA) was used to crosslink pADM, and highly hydrophilic and moisturizing hyaluronic acid (HA) molecules were introduced into pADM to prepare the EHA-crosslinked acellular porcine dermal matrix (EHA-pADM). FTIR spectrometer, AFM, shrinkage temperature tester and differential scanning calorimeter were used to analyze the EHA-pADM crosslinked with different dosages of EHA. Results indicated that the thermal stability of EHA-pADM increased with the increase of the amount of EHA, resulting in a cross-linking effect, but EHA-pADM still maintained the original secondary structure, triple helix structure, transeverse structure and other microstructure characteristics of pADM. The surface contact angle of EHA-pADM decreases with the increase of the amount of EHA, and its moisture absorption rate and water retention rate were basically positively correlated with the amount of EHA. Moisture absorption kinetic analysis showed that the amount of water adsorbed by EHA-pADM increased with the increase of the amount of EHA, and the adsorption characteristics were in line with the second-order kinetic equation, and the adsorption process was a multi-layer adsorption. It can be seen that the introduction of HA molecules improves the surface hydrophilicity of pADM and increases its water-retention and "water-locking" ability. When EHA-pADM acts as a dressing, it is expected to become a new type of hydrophilic and moisturizing medical dressing.

Key words: epoxidized hyaluronic acid, acellular dermal porcine matrix, cross-linking, hydrophilicity, moisturizing, biological dressing

中图分类号:

TS513

丁壮, 黄轩涛, 但年华. 环氧透明质酸改性脱细胞猪真皮基质的亲水保湿性研究[J]. 皮革科学与工程, 2022, 32(3): 18-23.

DING Zhuang, HUANG Xuantao, DAN Nianhua. Study on the Hydrophilicity and Moisturizing of Acellular Porcine Dermal Matrix Crosslinked by Epoxidized Hyaluronic Acid[J]. Leather Science and Engineering, 2022, 32(3): 18-23.

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环氧透明质酸改性脱细胞猪真皮基质的亲水保湿性研究

Study on the Hydrophilicity and Moisturizing of Acellular Porcine Dermal Matrix Crosslinked by Epoxidized Hyaluronic Acid

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