胶原蛋白热变性检测方法的比较研究

doi:10.19677/j.issn.1004-7964.2021.03.008

皮革科学与工程 ›› 2021, Vol. 31 ›› Issue (3): 41-45.doi: 10.19677/j.issn.1004-7964.2021.03.008

胶原蛋白热变性检测方法的比较研究

李新莹¹, 缪可言², 葛黎明^*, 李德富^*

1. 西南民族大学化学与环境学院,四川成都 610041;
2. 四川大学化学工程学院,四川成都 610065

收稿日期:2020-09-22 出版日期:2021-06-28 上线日期:2021-05-28
通讯作者: 葛黎明,E-mail: geliming@scu.edu.cn;李德富, E-mail: lidefu@scu.edu.cn。
作者简介:李新莹（1984-）,女,博士,副教授,电话：028-85522114,E-mail: lixinying1984@163.com。
基金资助:
西南民族大学中央高校基本科研业务费专项资金项目（2018NQN07）

Comparative Study on Detection Methods of Thermal Denaturation of Collagen

LI Xinying¹, MIAO Keyan², GE Liming^2,*, LI Defu^2,*

1. School of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China;
2. School of Chemical Engineering, Sichuan University, Chengdu 610065, China

Received:2020-09-22 Online:2021-06-28 Published:2021-05-28

摘要/Abstract

摘要： 采用圆二色光谱法、旋光度法、傅立叶变换红外光谱法、超灵敏差示扫描量热法以及X-射线衍射法对Ⅰ型胶原蛋白的热变性进行了检测,以探究胶原蛋白热变性不同检测方法的差异。结果表明,上述不同方法都是研究胶原蛋白热变性的有力工具,圆二色光谱法、旋光度法和超灵敏差示扫描量热法可以监测溶液中胶原蛋白的热变性过程,傅立叶变换红外光谱法和X-射线衍射法可以定性检测固体状态胶原蛋白的热变性。其中,超灵敏差示扫描量热法和圆二色光谱法与其他方法相比灵敏度更高。本论文结果将为胶原蛋白的相关研究提供参考。

关键词: 胶原蛋白, 热变性, 测试方法

Abstract: The thermal denaturation of type I collagen was studied by circular dichroism (CD), polarimetry, Fourier transform infrared spectroscopy (FTIR), ultrasensitive microcalorimetry (US-DSC) and X-ray diffraction (XRD). The results indicated that the above methods were the powerful tools to study the thermal denaturation of collagen. The process of thermal denaturation of collagen in solution could be monitored by CD, polarimetry and US-DSC. And the thermal denaturation of solid state collagen could be detected qualitatively by FTIR and XRD. Among them, US-DSC and CD were more sensitive than other methods. The results will provide a theoretical basis for the related research of collagen.

Key words: collagen, thermal denaturation, detection method

中图分类号:

TS57

李新莹, 缪可言, 葛黎明, 李德富. 胶原蛋白热变性检测方法的比较研究[J]. 皮革科学与工程, 2021, 31(3): 41-45.

LI Xinying, MIAO Keyan, GE Liming, LI Defu. Comparative Study on Detection Methods of Thermal Denaturation of Collagen[J]. Leather Science and Engineering, 2021, 31(3): 41-45.

参考文献

[1] 高玲玲,侯成立,高远,等.胶原蛋白热稳定性研究进展[J].中国食品学报,2018,18(5):195-207.
[2] Duan S M, Wang W H, Li S Z, et al.Moderate laccase-crosslinking improves the mechanical and thermal properties of acid-swollen collagen-based films modified by gallotannins[J]. Food Hydrocolloids, 2020, 106: 105917.
[3] 胡可欣,李国英.没食子酸对胶原蛋白膜的改性研究[J].皮革科学与工程,2020,30(4):1-6.
[4] Sionkowska A, Walczak M, Michalska-Sionkowska M.Preparation and characterization of collagen/chitosan composites with silver nanoparticles[J]. Polymer Composites, 2020, 41(3): 951-957.
[5] Kozlowska J, Stachowiak N, Sionkowska A.Collagen/gelatin/hydroxyethyl cellulose composites containing microspheres based on collagen and gelatin: design and evaluation[J]. Polymers, 2018, 10(4): 456.
[6] 朱欣星,安然,李昌朋,等.胶原与明胶的结构研究:方法、结果与分析[J].皮革科学与工程,2012,22(5):9-14.
[7] Zhang C,Yang X S, Hu W Q, et al.Preparation and characterization of carboxymethyl chitosan/collagen peptide/oxidized konjac composite hydrogel[J]. International Journal of Biological Macromolecules, 2020, 149: 31-40.
[8] Akita M, Nishikawa Y, Shigenobu Y, et al.Correlation of proline, hydroxyproline and serine content, denaturation temperature and circular dichroism analysis of type I collagen with the physiological temperature of marine teleosts[J]. Food Chemistry, 2020, 329: 126775.
[9] Cheng S,Wang W H, Li Y, et al.Cross-linking and film-forming properties of transglutaminase-modified collagen fibers tailored by denaturation temperature[J]. Food Chemistry, 2019, 271: 527-535.
[10] Kojima C, Nishio M, Nakajima Y, et al.Rapid photoinduced single cell detachment from gold nanoparticle-embedded collagen gels with low denaturation temperature[J]. Polymers, 2020, 12(1): 213.
[11] Latorre M E, Velazquez D E, Purslow P P.Differences in the energetics of collagen denaturation in connective tissue from two muscles[J]. International Journal of Biological Macromolecules, 2018, 113: 1294-1301.
[12] Mu C D, Li D F, Lin W, et al.Temperature induced denaturation of collagen in acidic solution[J]. Biopolymers, 2007, 86(4): 282-287.
[13] 李德富,周玉玲,穆畅道,等.超声波对溶液中胶原蛋白聚集体和三股螺旋结构的影响[J].皮革科学与工程,2009,19(5):14-17.
[14] Hwang J M,San B H,Turner N J,et al.Molecular assessment of collagen denaturation in ecellularized tissues using a collagen hybridizing peptide[J]. Acta Biomaterialia,2017,53:268-278.
[15] Li D F, Mu C D, Zhang Q, et al.Effectsofmicrowave irradiation on collagendenaturation[J]. Journal of Food Biochemistry, 2010, 34(6): 1319-1331.
[16] Cadenaro M, Fontanive L, Navarra C O, et al.Effect of carboidiimide on thermal denaturation temperature of dentin collagen[J]. Dental Materials, 2016, 32(4): 492-498.
[17] 张奇,唐春雪,丁克毅,等.光谱分析在改性胶原蛋白结构表征中的应用(II)——X射线衍射和核磁共振[J].中国皮革,2019,48(9):23-28.
[18] Wu B, Mu C D, Zhang G Z, et al.Effects of Cr³+ on the structure of collagen fiber[J]. Langmuir, 2009, 25(19): 11905-11910.

胶原蛋白热变性检测方法的比较研究

Comparative Study on Detection Methods of Thermal Denaturation of Collagen

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