高通量测序对不同工艺厌氧处理下污泥微生物的群落分析

doi:10.19677/j.issn.1004-7964.2021.04.004

皮革科学与工程 ›› 2021, Vol. 31 ›› Issue (4): 17-21.doi: 10.19677/j.issn.1004-7964.2021.04.004

高通量测序对不同工艺厌氧处理下污泥微生物的群落分析

常敏^1,2,3,4, 马宏瑞⁵

1.陕西省土地工程建设集团有限责任公司,陕西西安 710075;
2.自然资源部退化及未利用土地整治工程重点实验室,陕西西安 710021;
3.陕西地建土地工程技术研究院有限责任公司,陕西西安 710021;
4.陕西省土地整治工程技术研究中心,陕西西安 710075;
5.陕西科技大学环境科学与工程学院,陕西西安 710021

收稿日期:2021-02-02 出版日期:2021-08-28 上线日期:2021-08-02
作者简介:常敏（1992-）,女,陕西西安人,硕士,主要研究方向为土地工程、工业水处理等,E-mail：526885826@qq.com。

High-throughput Sequencing Analysis of Sludge Microbial Communities under Different Anaerobic Treatment Processes

CHANG Min^1,2,3,4, MA Hongrui⁵

1. Shaanxi Provincial Land Engineering Construction Group Co.,Ltd., Xi'an 710075, China;
2. Key Laboratory of Degraded and Unused Land Consolidation Engineering, the Ministry of Natural Resources, Xi'an 710021, China;
3. Institute of Land Engineering and Technology, Shaanxi Provincial Land Engineering Construction Group Co.,Ltd., Xi'an 710021, China;
4. Shaanxi Provincial Land Consolidation Engineering Technology Research Center, Xi'an 710075, China;
5. School of Environmental Science and Engineering,Shaanxi University of Science & Technology, Xi'an 710021,China

Received:2021-02-02 Published:2021-08-28 Online:2021-08-02

摘要/Abstract

摘要： 为研究工业废水处理领域IC厌氧反应器和水解酸化池工艺处理下污泥微生物群落结构,本研究通过Illumina MiSeq平台对不同工艺厌氧污泥16S rDNA高变区V3~V4区进行测序分析。水解酸化池污泥中优势菌群为变形菌门(Proteobacteria),而IC反应器颗粒污泥里主要的优势菌门主要为拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）,变形菌门(Proteobacteria)所占比例大幅降低,推测在厌氧处理废水过程中致病菌大幅减少,IC反应器对该水质有显著的微生物净化作用。此外,IC反应器污泥中出现一定比例的古生菌门（Archaea）,产甲烷菌较为活跃,且在运行过程中形成了特定的菌群结构,所占比例较稳定,利于反应进行,可在IC反应器处理毛皮废水厌氧产甲烷体系的微生物快速构建及优化方面提供理论指导。

关键词: 高通量测序, 厌氧, 污泥, 微生物, 群落

Abstract: In order to study the sludge microbial community structure under the IC anaerobic reactor and hydrolysis acidification tank process in the industrial wastewater treatment field, the 16S rDNA hypervariable region V3~V4 of anaerobic sludge from different processes were analyzed by employing the Illumina MiSeq platform. The dominant bacteria group in the sludge of the hydrolysis acidification tank is the Proteobacteria, and the main dominant bacteria in the granular sludge of the IC reactor are Bacteroidetes and Firmicutes. The proportion of Proteobacteria is greatly reduced. It is speculated that pathogenic bacteria will be greatly reduced in the process of anaerobic treatment of wastewater, and the IC reactor has a significant microbial purification effect on the water quality. In addition, a certain proportion of Archaea appears in the sludge of the IC reactor, and methanogens are more active, and a specific bacterial microbial community structure is formed during operation. The proportion is relatively stable, which is conducive to the reaction. Results presented in this manuscript provide theoretical guidance on the rapid construction and optimization of microorganisms in the anaerobic methane production system for treating fur wastewater in the IC reactor.

Key words: High-throughput sequencing, anaerobic, sludge, microorganism, community

中图分类号:

X794

常敏, 马宏瑞. 高通量测序对不同工艺厌氧处理下污泥微生物的群落分析[J]. 皮革科学与工程, 2021, 31(4): 17-21.

CHANG Min, MA Hongrui. High-throughput Sequencing Analysis of Sludge Microbial Communities under Different Anaerobic Treatment Processes[J]. Leather Science and Engineering, 2021, 31(4): 17-21.

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高通量测序对不同工艺厌氧处理下污泥微生物的群落分析

High-throughput Sequencing Analysis of Sludge Microbial Communities under Different Anaerobic Treatment Processes

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