非生物腐殖化的微生物生理效应和产物转化

doi:10.19677/j.issn.1004-7964.2024.03.003

皮革科学与工程 ›› 2024, Vol. 34 ›› Issue (3): 17-24.doi: 10.19677/j.issn.1004-7964.2024.03.003

非生物腐殖化的微生物生理效应和产物转化

朱超¹, 周明芳¹, 利浩男¹, 马宏瑞^1,*, 郝永永²

1.陕西科技大学环境科学与工程学院,陕西西安 710021;
2.陕西科技大学轻工科学与工程学院,陕西西安 710021

收稿日期:2023-10-31 修回日期:2023-12-13 接受日期:2023-12-15 出版日期:2024-06-01 上线日期:2024-04-25
通讯作者: *马宏瑞（1963-）,男,博士,教授,主要研究方向:化工废水处理技术与工程应用研究。E-mail:mahr@sust.edu.cn。
作者简介:朱超（1982-）,男,博士,副教授,主要研究方向:环境微生物及环境污染治理。E-mail:304545693@qq.com。
基金资助:
国家水污染防治重大科技计划项目（2017ZX07602-001）; 国家自然科学基金项目（22076113）

Microbial Physiological Effects and Product Transformation of Abiotic Humification

ZHU Chao¹, ZHOU Mingfang¹, LI Haonan¹, MA Hongrui^1,*, HAO Yongyong²

1. College of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;
2. College of Light Industry Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China

Received:2023-10-31 Revised:2023-12-13 Accepted:2023-12-15 Published:2024-06-01 Online:2024-04-25

摘要/Abstract

摘要： 制革废水中的可溶性有机物在生物处理系统的转化行为还不清楚。以单宁酸、甘氨酸和葡萄糖为原料模拟废水发生非生物腐殖化,并结合多元光谱技术,探究了非生物腐殖化产物在微生物纯培养和混合培养中的转化及对生物代谢活性的影响。结果表明:单宁酸能促进非生物腐殖化进程,同时经非生物腐殖化体系暴露后,纯培养在48 h-1 000 mg/L氨基酸类区域的荧光强度增强;混合培养在96 h-2 000 mg/L类腐殖酸区荧光强度最强,并新增了类溶解性微生物副产物的荧光区。衡量腐殖化程度的SUVA₂₅₄值分析显示,混合培养的SUVA₂₅₄值是纯培养的3倍,说明非生物腐殖化产物经混合培养转化后芳香性显著提升。研究结果揭示了非生物腐殖化和其生物转化产物是制约制革废水达标处理的重要控制因素。

关键词: 溶解性有机物, 非生物腐殖化, 大肠杆菌, 活性污泥

Abstract: The transformation behavior of dissolved organic matter in tannery wastewater is still unclear in biological treatment systems. Herein, tannic acid, glycine and glucose were used as raw materials to simulate the abiotic humification of wastewater. By combining with multivariate spectroscopy, we investigated the characteristics of the abiotic humus products, the transformation and the effects on the metabolic activity of microorganisms in both pure and mixed culture systems. Results showed that tannic acid promoted the abiotic humification process, while the fluorescence intensity of the amino acid-like region was enhanced at 48 h-1 000 mg/L in the pure culture after the microorganisms were exposed to the abiotic humification system. The fluorescence intensity was the strongest in the humic acid-like region at 96 h-2 000 mg/L in the mixed culture, and the fluorescence region of the solubilized microbial by-product-like products was added. Analysis of SUVA₂₅₄, a measure of the degree of humification, showed that the mixed culture was three times higher than the pure culture, indicating a significant increase in the aromaticity of the transformed abiotic humic products compared to the mixed culture. This work revealed that abiotic humification and its biotransformation products were important control factors governing the standardized treatment of tannery wastewater.

Key words: dissolved organic matter, abiotic humification, Escherichia coli, activated sludge

中图分类号:

朱超, 周明芳, 利浩男, 马宏瑞, 郝永永. 非生物腐殖化的微生物生理效应和产物转化[J]. 皮革科学与工程, 2024, 34(3): 17-24.

ZHU Chao, ZHOU Mingfang, LI Haonan, MA Hongrui, HAO Yongyong. Microbial Physiological Effects and Product Transformation of Abiotic Humification[J]. Leather Science and Engineering, 2024, 34(3): 17-24.

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非生物腐殖化的微生物生理效应和产物转化

Microbial Physiological Effects and Product Transformation of Abiotic Humification

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