皮革科学与工程 ›› 2021, Vol. 31 ›› Issue (2): 16-22.doi: 10.19677/j.issn.1004-7964.2021.02.004

• 综述 • 上一篇    下一篇

微生物胶原酶研究进展

王学川1, 王雪莹1, 杨淑琴2, 何芳芳1   

  1. 1.陕西科技大学轻工科学与工程学院生物质与功能材料研究所,陕西 西安 710021;
    2.淄博大桓九宝恩皮革集团有限公司,山东 淄博 255000
  • 收稿日期:2020-07-02 出版日期:2021-04-28 上线日期:2021-03-30
  • 作者简介:王学川(1963-),男,博士,教授,博士生导师,E-mail:wangxc@sust.edu.cn. 研究方向:清洁生产。
  • 基金资助:
    国家自然科学基金项目(21776169)

A Review: Microbial Collagenase

WANG Xuechuan1, WANG Xueying1, YANG Shuqin2, HE Fangfang1   

  1. 1. Institute of Biomass & Functional Materials , College of Bioresources Chemical and Material Engineering, Shaanxi University of Science & Technology, Xi’an, 710021, China;
    2. Zibo Dahuanjiu Polygrace Leather Group Co. Ltd., Zibo 255000, China
  • Received:2020-07-02 Online:2021-04-28 Published:2021-03-30

摘要: 胶原酶具有很强的特异性,其降解胶原的能力在动物细胞外基质的整体降解中起着关键作用。首先介绍了微生物胶原酶的分类和结构,简述了目前微生物胶原酶活性测定、胶原酶基因克隆、表达和纯化表征的技术及方法,然后综述了胶原酶在皮革工业、医药工业和细胞分离等领域的应用及其前景,最后对胶原酶现存在的问题也做了简要的概括,并对未来的发展方向进行了展望。

关键词: 微生物, 胶原酶, 分类, 应用, 研究进展

Abstract: Collagenase is highly specific and plays a key role in the overall degradation of animal extracellular matrix due to its ability to degrade collagen. This review introduced the classification and structure of collagenase, and expounded the techniques and methods used in the activity determination, gene cloning, expression, purification and characterization of microbial collagenase. The application and prospect of collagenase was then summarized in leather industry, medical industry, cell separation and other fields. Finally, the existing problems for collagenase were briefly summarized, and the future development direction was prospected.

Key words: microorganism, collagenase, classification, application, research progress

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