胶原静电纺丝用水性溶剂体系对鱼胶原结构的影响

doi:10.19677/j.issn.1004-7964.2019.06.001

皮革科学与工程 ›› 2019, Vol. 29 ›› Issue (06): 5-10.doi: 10.19677/j.issn.1004-7964.2019.06.001

• 试验研究 • 下一篇

胶原静电纺丝用水性溶剂体系对鱼胶原结构的影响

刘文涛^1,2*, 龚丽珂¹, 郭正国¹

1.四川大学皮革化学与工程教育部重点实验室,四川成都 610065;
2.四川大学制革清洁技术国家工程实验室,四川成都 610065

收稿日期:2019-09-15 出版日期:2019-12-28 上线日期:2020-03-27
作者简介:刘文涛(1982-),男,博士,副教授;研究方向:胶原基功能材料设计及应用。E-mail:liuwt@scu.edu.cn。
基金资助:
国家自然科学基金(21606156)

Effects of Water-based Solvents used for Collagen Electrospinning on the Structure of Fish Collagen

LIU Wentao^1,2*, GONG Like¹, GUO Zhengguo¹

1. Key Laboratory of Leather Chemistry and Engineering Sichuan University, Ministry of Education, Chengdu 610065, China;
2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China

Received:2019-09-15 Online:2019-12-28 Published:2020-03-27

摘要/Abstract

摘要： 研究了两类胶原电纺用水性溶剂体系(醋酸溶液和PBS/乙醇混合溶剂)对黑鱼皮胶原结构的影响。首先分析了文献报道水性溶剂体系的稳定性与表面张力,在此基础上分别用3个浓度的醋酸溶液(0.5 mol/L、1 mol/L和2 mol/L)及3个配比的PBS/乙醇混合溶剂(V(PBS)∶V(乙醇) 为3∶1、3∶0.5和3∶0.25)溶解鱼皮胶原制备胶原溶液,综合运用电泳、红外和紫外光谱以及超灵敏差示扫描量热等技术手段考察了不同鱼皮胶原溶液的结构性质。结果表明,两类水性溶剂体系随着醋酸或乙醇浓度的增加,都对鱼胶原的结构稳定性产生破坏,而且乙醇对鱼胶原的变性作用更强;醋酸溶液可能比PBS/乙醇混合溶剂更适合用于鱼胶原的连续稳定电纺,但有必要采取策略同步增强胶原的结构稳定性与静电可纺性。

关键词: 鱼胶原, 静电纺丝, 水性溶剂, 结构稳定性, 变性

Abstract: The effects of two kinds of water-based solvents used for collagen electrospinning (acetic acid solution and PBS/ethanol mixed solvent) on the structure of snakehead skin collagen were studied. Firstly, the stability and surface tension of the water-based solvents reported in literatures were analyzed. On this basis, three concentrations (0.5 mol/L, 1 mol/L, and 2 mol/L) of acetic acid solution and three types of PBS/ethanol mixed solvent (V(PBS)∶V(ethanol) equals to 3∶1, 3∶0.5, and 3∶0.25) were used to prepare fish collagen solutions. The techniques including electrophoresis, infrared and ultraviolet spectroscopy and ultra-sensitive differential scanning calorimetry were used to study the structural properties of different fish skin collagen solutions. Results showed that the two kinds of water-based solvents destroyed the structural stability of fish collagen with the increase of acetic acid or ethanol concentration, and the denaturation effect of ethanol on fish collagen was stronger. Acetic acid solution maybe more suitable for continuous stable electrospinning of fish collagen than PBS/ethanol mixed solvent, but it is necessary to take a strategy to simultaneously enhance the structural stability and electrostatic spinnability of collagen.

Key words: fish collagen, electrospinning, water-based solvents, structural stability, denaturation

中图分类号:

TS512

刘文涛, 龚丽珂, 郭正国. 胶原静电纺丝用水性溶剂体系对鱼胶原结构的影响[J]. 皮革科学与工程, 2019, 29(06): 5-10.

LIU Wentao, GONG Like, GUO Zhengguo. Effects of Water-based Solvents used for Collagen Electrospinning on the Structure of Fish Collagen[J]. Leather Science and Engineering, 2019, 29(06): 5-10.

参考文献

[1] Wade RJ, Burdick JA.Advances in nanofibrous scaffolds for biomedical applications: From electrospinning to self-assembly[J]. Nano Today, 2015, 9: 722-742.
[2] 王策,卢晓峰.有机纳米功能材料:高压静电纺丝技术与纳米纤维[M].北京:科学出版社,2011.
[3] 李国英,刘文涛.胶原化学[M].北京:中国轻工业出版社,2013.
[4] 黄艳萍,但年华,但卫华.静电纺丝制备胶原基复合纳米医用纤维的研究进展[J].材料导报,2019,33(10):3322-3327.
[5] Lin K,Zhang D,Macedo MH,et al.Advanced collagen-based biomaterials for regenerative biomedicine[J].Advanced Functional Materials,2019,1804943.
[6] 瞿怡,苏寒雨,刘文涛,等.超声波辅助法提取牛皮胶原蛋白[J].皮革科学与工程,2018,28(2):5-9.
[7] 李倩,杨欢,李国英.[EMIM][AC]溶剂对Ⅰ型胶原结构的影响[J].皮革科学与工程,2017,27(5):5-9.
[8] Liu W,Zhang Y,Cui N,et al.Extraction and characterization of pepsin-solubilized collagen from snakehead(Channa argus)skin:Effects of hydrogen peroxide pretreatments and pepsin hydrolysis strategies[J].Process Biochemistry,2019,76:194-202.
[9] 孔丽丽,巩凡,Plavan V,等.酸酶复合法提取鲢鱼鱼鳞胶原蛋白的工艺研究[J].皮革科学与工程,2014,24(4):11-15.
[10] Matthews JA,Wnek GE,Simpson DG,et al.Electrospinning of collagen nanofibers[J].Biomacromolecules,2002,3:232-238.
[11] Zeugolis DI,Khew ST,Yew ESY,et al.Electro-spinning of pure collagen nano-fibres-Just an expensive way to make gelatin[J].Biomaterials,2008,29:2293-2305.
[12] Dong B,Arnoult O,Smith ME,et al.Electrospinning of collagen nanofiber scaffolds from benign solvents[J].Macromolecular Rapid Communications,2009,30:539-542.
[13] Dong Z,Wu Y,Clark RL.Thermodynamic modeling and investigation of the formation of electrospun collagen fibers[J].Langmuir,2011,27:12417-12422.
[14] Hofman K,Tucker N,Stanger J,et al.Effects of the molecular format of collagen on characteristics of electrospun fibres[J].Journal of Materials Science,2012,47:1148-1155.
[15] Bürck J,Heissler S,Geckle U,et al.Resemblance of electrospun collagen nanofibers to their native structure[J].Langmuir,2013,29:1562-1572.
[16] Fiorani A,Gualandi C,Panseri S,et al.Comparative performance of collagen nanofibers electrospun from different solvents and stabilized by different crosslinkers[J].Journal of Materials Science:Materials in Medicine,2014,25:2313-2321.
[17] Kazanci M.Solvent and temperature effects on folding of electrospun collagen nanofibers[J].Materials Letters,2014,130:223-226.
[18] Shanmguam G,Reddy SMM,Natarajan V,et al.2,2,2-Trifluoroethanol disrupts the triple helical structure and self-association of type I collagen[J].International Journal of Biological Macromolecules,2013,54:155-159.
[19] 郑少东,胡杨,但年华,等.六氟异丙醇对I型胶原分子结构完整性的影响[J].中国皮革,2014,43(17):23-26.
[20] Liu W,Li G.Non-isothermal kinetic analysis of the thermal denaturation of type I collagen in solution using isoconversional and multivariate non-linear regression methods[J].Polymer Degradation and Stability,2010,95:2233-2240.
[21] Liu W,Tian Z,Li C,et al.Thermal denaturation of fish collagen in solution:A calorimetric and kinetic analysis[J].Thermochimica Acta,2014,581:32-40.

胶原静电纺丝用水性溶剂体系对鱼胶原结构的影响

Effects of Water-based Solvents used for Collagen Electrospinning on the Structure of Fish Collagen

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