发光细菌法评价坯革生态安全性的毒性参照物替代及应用

doi:10.19677/j.issn.1004-7964.2022.03.003

皮革科学与工程 ›› 2022, Vol. 32 ›› Issue (3): 13-17.doi: 10.19677/j.issn.1004-7964.2022.03.003

发光细菌法评价坯革生态安全性的毒性参照物替代及应用

何发明¹, 彭良琼², 隆汶君², 张文华^1,2,*

1.四川大学皮革化学与工程教育部重点实验室,四川成都 610065;
2.四川大学制革清洁技术国家工程实验室,四川成都 610065

收稿日期:2022-01-15 出版日期:2022-06-28 上线日期:2022-05-16
通讯作者: ^*zhangwh@scu.edu.cn
作者简介:何发明（1995-）,男,籍贯四川,硕士研究生,研究方向为生物质综合利用,E-mail：1264594449@qq.com。
基金资助:
国家自然科学基金（21776185）; 国家重点研发项目（2017YFB0308503)

Substitution and Application of Toxicity Reference Substance for Evaluating the Ecological Safety of Leather by Luminescent Bacteria

HE Faming¹, PENG Liangqiong², LONG Wenjun², ZHANG Wenhua^1,2,*

1. Key Laboratory of Leather Chemistry and Engineer of Ministry of Education, Sichuan University, Chengdu 610065, China;
2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China

Received:2022-01-15 Online:2022-06-28 Published:2022-05-16

摘要/Abstract

摘要： 针对发光细菌法常用的毒性测试参照物氯化汞,选取毒性较小的锌盐作为参比物质替代,研究了SO₄^2-和Cl^-对锌的形态分布、稳定性以及毒性的影响。结果表明,在水溶液中,无机阴离子对锌的形态分布影响较小;在毒性测试的3% NaCl介质中,虽然硫酸锌形态成分较氯化锌多,但在pH 1~8范围内组成恒定,主要为65%的Zn²⁺和25%的[ZnCl]⁺。硫酸锌存放稳定性更好,而氯化锌易吸湿;硫酸锌对发光细菌的EC₅₀值为1.23 mg/L,大于氯化锌的EC₅₀值0.92 mg/L,毒性更低。最后,以硫酸锌为参比物,评价了常见鞣剂鞣制坯革的生态安全性,根据现有法令关于危废的综合毒性指标,发现所有皮坯都可归属为普通废弃物,但铬比有机鞣剂更影响鞣制皮坯的生态安全性。

关键词: 发光细菌, 参比物质, 硫酸锌, 坯革, 生态性评价

Abstract: HgCl₂ was commonly used as toxicity reference substance in the luminescent bacteria test. In this paper, low toxic zinc salts were used as a substitution by studying the effects of SO₄^2- and Cl^- on the species distribution, stability and toxicity of Zn²⁺. The results showed that in aqueous solution, inorganic anions had little effect on the species of zinc. In 3% NaCl medium of the toxicity test, more Zn²⁺ species in zinc sulfate was observed. Nevertheless, the content remained constant mainly 65% Zn²⁺ and 25% [ZnCl]⁺ in the pH range of 1-8. Zinc sulfate had better storage stability, while zinc chloride was easy to absorb moisture. The EC₅₀ value of zinc sulfate was 1.23 mg/L greater than 0.92 mg/L of zinc chloride. Finally, using zinc sulfate as a reference, the ecological safety of leather tanned by common tanning agents was evaluated. The results showed that all leather samples satisfied the comprehensive toxicity threshold as common waste. However, chromium affected the ecological safety of tanned leather to some extent compared with organic tanning agents.

Key words: luminescent bacteria, reference substance, zinc sulfate, leather, ecological evaluation

中图分类号:

TS57

何发明, 彭良琼, 隆汶君, 张文华. 发光细菌法评价坯革生态安全性的毒性参照物替代及应用[J]. 皮革科学与工程, 2022, 32(3): 13-17.

HE Faming, PENG Liangqiong, LONG Wenjun, ZHANG Wenhua. Substitution and Application of Toxicity Reference Substance for Evaluating the Ecological Safety of Leather by Luminescent Bacteria[J]. Leather Science and Engineering, 2022, 32(3): 13-17.

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发光细菌法评价坯革生态安全性的毒性参照物替代及应用

Substitution and Application of Toxicity Reference Substance for Evaluating the Ecological Safety of Leather by Luminescent Bacteria

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