发光细菌法评价坯革的生态毒性

doi:10.19677/j.issn.1004-7964.2021.06.001

皮革科学与工程 ›› 2021, Vol. 31 ›› Issue (6): 1-7.doi: 10.19677/j.issn.1004-7964.2021.06.001

• 试验研究 • 下一篇

发光细菌法评价坯革的生态毒性

彭良琼¹, 隆汶君¹, 何发明¹, 王睿², 张文华^1,2,*, 石碧^1,2

1.四川大学皮革化学与工程教育部重点实验室,四川成都 610065;
2.四川大学制革清洁技术国家工程研究中心,四川成都 610065

收稿日期:2021-07-20 出版日期:2021-12-28 上线日期:2021-11-25
通讯作者: *zhangwh@scu.edu.cn
作者简介:彭良琼（1993-）,女,四川仁寿人,博士研究生,研究方向为生物质化学与工程,E-mail：995097453@qq.com。
基金资助:
国家自然科学基金（21776185）; 国家重点研发计划（2017YFB0308503）

Evaluating Ecotoxicity of Leather by Luminescent Bacteria

PENG Liangqiong¹, LONG Wenjun¹, HE Faming¹, WANG Rui², ZHANG Wenhua^1,2,*, SHI Bi^1,2

1. Key Laboratory of Leather Chemistry and Engineer of Ministry of Education, Sichuan University, Chengdu 610065, China;
2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China

Received:2021-07-20 Online:2021-12-28 Published:2021-11-25

摘要/Abstract

摘要： 皮革产业在我国国民经济体系中占重要地位,其生产过程产生的革屑和废弃革制品也可能带来环境风险。本文首先针对皮革行业关注的六价铬、甲醛、五氯苯酚、四氯苯酚、邻苯基苯酚、以及壬基酚,研究了这些有害物质的物种敏感性。比较分析表明,发光细菌对六种物质均具有较高的毒性测试敏感度。从市场抽样四种鞣法的染整坯革,与去离子水在固/液比1∶20 g/mL、100 r/min、25 ℃浸提8 h,以明亮发光杆菌测试浸出液EC₅₀值,发现铬鞣、有机鞣、植鞣、锆铝鞣坯革分别为21.46%、26.76%、49.50%和88.56%,表明各鞣法坯革生态毒性：铬鞣>有机鞣>植鞣>锆铝鞣,铬鞣革显示出较大的负面生态效应。本研究表明,发光细菌能够定量区分不同坯革的生态性,为坯革的综合生态性评价提供了一种方便、可行的方法。

关键词: 坯革, 有害物质, 敏感性分析, 发光细菌, 综合生态毒性

Abstract: Leather industry plays an important role in our national economy, whereas both leather shavings and discarded leather products may pose potential environmental risks. This paper studied the species sensitivity of hexavalent chromium, formaldehyde, pentachlorophenol, tetrachlorophenol, o-phenylphenol, and nonylphenol which are hazardous substances concerned by global leather industry. The comparative analysis showed that luminescent bacteria had high sensitivity to these six substances. Then four types of dyed crust leather were collected from different factories and extracted with deionized water at a solid/liquid ratio of 1∶20 g/mL, 100 rpm, and 25 ℃ for 8 h. The ecotoxicity of the leachates was tested with Photobacterium phosphoreum, and the EC₅₀ values of the crust leather tanned by chrome, organics, vegetable tannin, and zirconium-aluminum were 21.46%, 26.76%, 49.50%, and 88.56%, respectively, indicating the ecotoxicity of crust leathers followed the sequence based on tanning agent: chrome > organics > vegetable tannin > zirconium-aluminum, chrome tanned crust leather showed a large negative ecological effect. This study showed that luminescent bacteria can distinguish the ecotoxicity of different leathers, which provides a convenient and feasible method for comprehensive ecological evaluation of leather.

Key words: leather, hazardous substances, sensitivity analysis, luminescent bacteria, ecotoxicity

中图分类号:

TS57

彭良琼, 隆汶君, 何发明, 王睿, 张文华, 石碧. 发光细菌法评价坯革的生态毒性[J]. 皮革科学与工程, 2021, 31(6): 1-7.

PENG Liangqiong, LONG Wenjun, HE Faming, WANG Rui, ZHANG Wenhua, SHI Bi. Evaluating Ecotoxicity of Leather by Luminescent Bacteria[J]. Leather Science and Engineering, 2021, 31(6): 1-7.

参考文献

[1] 卢欣雨,刘健西.环境规制及产业升级视角下的皮革产业发展环境分析[J].皮革科学与工程,2020,30(06):84-88.
[2] Covington A D, Khan M I, Cheema S A, et al.Modern tanning chemistry[J]. Chemical Society Reviews, 1997, 26(2): 111-26.
[3] Sundar V J, Muralidharan C, Mandal A B.A novel chrome tanning process for minimization of total dissolved solids and chromium in effluents[J]. Journal of Cleaner Production, 2013, 59: 239-44.
[4] Pati A, Chaudhary R, Subramani S.A review on management of chrome-tanned leather shavings: a holistic paradigm to combat the environmental issues[J]. Environmental Science and Pollution Research, 2014, 21(19): 11266-82.
[5] Peng L Q, Li Y, Han W M, et al.Investigation into the hazards of finished Cr-tanned leather as both product and waste[J]. Journal of the Society of Leather Technologists and Chemists, 2019, 103(1): 43-8.
[6] Dixit S, Yadav A, Dwivedi P D, et al.Toxic hazards of leather industry and technologies to combat threat: a review[J]. Journal of Cleaner Production, 2015, 87: 39-49.
[7] Dhayalan K, Fathima N N, Gnanamani A, et al.Biodegradability of leathers through anaerobic pathway[J]. Waste Management, 2007, 27(6): 760-7.
[8] 徐腾,南丰,蒋晓锋,等.制革场地土壤和地下水中铬污染来源及污染特征研究进展[J].土壤学报,2020,57(06):1341-52.
[9] 桑军,王梦頔,俞凌云,等.皮革行业化学品的管理现状[J].生态毒理学报,2015,10(02):123-30.
[10] 罗茜,马宏瑞,罗清拔,等.皮革化学品的环境风险评价及环境风险管理体系的建立[J].中国皮革,2014,43(07):36-40.
[11] 黄鹏,魏德东,赵青,等.四种水体修复氧化剂生物安全评估[J].环境生态学,2021,3(04):27-32.
[12] 谭杰峰,陈山多,王依钒,等.城镇污水处理厂排水对大型溞早期发育阶段的毒性效应[J].湖南农业科学,2020,(11):51-3+7.
[13] 胡丽,陈敏,殷高方,等.基于藻类光合作用抑制效应的敌草隆毒性响应参数研究[J].光谱学与光谱分析,2021,41(05):1519-24.
[14] 席佳锐,吴玲玲,付融冰,等.土壤环境中锑的生物毒性评价方法比较[J].环境监测管理与技术,2021,33(02):9-13.
[15] 马跃,宫琦,李术元,等.聚丙烯酰胺类油田添加剂生物毒性测试方法[J].沈阳工业大学学报:2021,1-6.
[16] 袁一鸣,王腾,何彦龙,等.大亚湾海域4种常见危化品对发光细菌的生物毒性研究[J].海洋环境科学,2021,40(02):235-41.
[17] Ambient water quality criteria forchromium [Z]//EPA. 440/5-80-035. 1980.
[18] Ambient water quality criteria for chlorinated phenols [Z]//EPA. 440/5-80-032. 1980.
[19] Ambient water quality criteria for pentachlorophenol [Z]//EPA. 440/5-80-065. 1980.
[20] Ambient water quality criteria for nonylphenol [Z]//EPA. EPA-882-R-05-005. 2005.
[21] Stouten H.Toxicological profile for o-phenylphenol and its sodium salt[J]. Journal of Applied Toxicology, 1998, 18(4): 261-70.
[22] Holmes G J, Eckert J W.Sensitivity of penicillium digitatum and p-italicum to postharvest citrus fungicides in california[J]. Phytopathology, 1999, 89(9): 716-21.
[23] Makinen P M, Theno T J, Ferguson J F, et al.Chlorophenol toxicity removal and monitoring in aerobic treatment: recovery from process upsets[J]. Environmental Science & Technology, 1993, 27(7): 1434-9.
[24] 周伟杰,韩威妺,彭良琼,等.pH对发光菌法测试Cr(Ⅲ)和Cr(Ⅵ)毒性的影响[J].皮革科学与工程,2017,27(06):5-9.
[25] Schuler L J,Hoang T C,Rand G M.Aquatic risk assessment of copper in freshwater and saltwater ecosystems of South Florida[J].Ecotoxicology,2008,17(7):642-59.
[26] 李真,苗晶晶,潘鲁青.6种重金属的发光菌毒性效应及其海洋生物物种敏感度分析[J].海洋环境科学,2015,34(02):176-83.
[27] 韩威妺,周萱,谭娟,等.发光细菌法评价典型制革表面活性剂的生态毒性[J].皮革科学与工程,2019,29(02):47-51+62.
[28] 张年国,潘桂平,周文玉.杂交东方鲀(菊黄♀×暗纹♂)幼鱼对4种常见药物的耐受性研究[J].水产科技情报,2020,47(02):00-5.
[29] Resendes A S, Dos Santos D S, Franca F M, et al. Acute toxic and genotoxic effects of formalin in danio rerio (zebrafish)[J]. Ecotoxicology, 2018, 27(10): 1379-86.
[30] Davoren M, Fogarty A M.Ecotoxicological evaluation of the biocidal agents sodium o-phenylphenol, sodium o-benzyl-p-chlorophenol, and sodium p-tertiary amylphenol[J]. Ecotoxicology and Environmental Safety, 2005, 60(2): 203-12.
[31] Soares A, Guieysse B, Mattiasson B.Biodegradation of nonylphenol in a continuous packed-bed bioreactor[J]. Biotechnology Letters, 2003, 25(12): 927-33.
[32] Gao K, Li B, Chen R, et al.A feasibility study of using silkworm larvae as a novel in vivo model to evaluate the biotoxicity of ionic liquids[J]. Ecotoxicology and Environmental Safety, 2021, 209: 111759.
[33] Singh A, Raj A, Padmanabhan A, et al.Combating silver nanoparticle-mediated toxicity in drosophila melanogaster with curcumin[J]. Journal of Applied Toxicology, 2020, 41(8): 1188-1199.
[34] Thurston R V, Gilfoil T A, Meyn E L, et al.Comparative toxicity of ten organic chemicals to ten common aquatic species[J]. Water Research, 1985, 19(9): 1145-55.
[35] 冯永亮. 淡水生物和海水生物从基因到种群水平指标对毒性物质的敏感性差异研究——以铜为例[J].生态毒理学报,2020,15(04):268-79.

发光细菌法评价坯革的生态毒性

Evaluating Ecotoxicity of Leather by Luminescent Bacteria

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	何发明, 彭良琼, 隆汶君, 张文华. 发光细菌法评价坯革生态安全性的毒性参照物替代及应用[J]. 皮革科学与工程, 2022, 32(3): 13-17.
[2]	何发明, 彭良琼, 林诗雨, 侯梦春, 张文华. 发光细菌法评价常用有机鞣剂的生态毒性[J]. 皮革科学与工程, 2021, 31(3): 14-18.
[3]	韩威妺;周萱;谭娟;彭良琼;张文华;. 发光细菌法评价典型制革表面活性剂的生态毒性[J]. , 2019, 29(02): 47-.
[4]	周伟杰;韩威妺;彭良琼;张文华;石碧;. pH对发光菌法测试Cr(Ⅲ)和Cr(Ⅵ)毒性的影响[J]. , 2017, 27(06): 5-9.
[5]	吕斌;段徐宾;高党鸽;马建中;. 亚硫酸化菜籽油/KH570-MMT纳米复合材料的制备[J]. , 2015, 25(02): 36-42.
[6]	蔡白雪;罗媛媛;饶剑;王豪;. 皮革制品有毒有害物质检测现状[J]. , 2014, 24(01): 27-30.
[7]	蔡白雪;罗媛媛;饶剑;王豪;. 皮革制品有毒有害物质检测现状[J]. , 2014, 24(01): 27-30.
[8]	林志勇;陈绍华;程群;林碧芬;. 我国皮革、毛皮及制品中部分有毒有害物质限量及检出[J]. , 2013, 23(01): 60-64.
[9]	石诗琦;董伟;罗杨;何有节;吴孟茹;. 皮革及其制品中有毒物质限量标准及部分检测方法[J]. , 2013, 23(01): 43-.
[10]	林志勇;陈绍华;程群;林碧芬;. 我国皮革、毛皮及制品中部分有毒有害物质限量及检出[J]. , 2013, 23(01): 60-64.
[11]	石诗琦;董伟;罗杨;何有节;吴孟茹;. 皮革及其制品中有毒物质限量标准及部分检测方法[J]. , 2013, 23(01): 43-.
[12]	陈斌;毛树禄;黄秋兰;. 鞋类产品中有毒有害物质的国外技术法规[J]. , 2009, 19(05): 54-58.
[13]	但卫华;曾睿;米贞建;. 皮革涂饰前坯革标准化建议[J]. , 2007, 17(06): 48-50.
[14]	但卫华,曾睿. 制革常见科技问题解答(11) 准备工段(1)[J]. , 2003, 13(06): 58-.
[15]	李林. 涂饰技术讲座(3) 一般涂饰技术[J]. , 2002, 12(05): 56-.