非生物腐殖化的微生物生理效应和产物转化

doi:10.19677/j.issn.1004-7964.2024.03.003

Leather Science and Engineering ›› 2024, Vol. 34 ›› Issue (3): 17-24.doi: 10.19677/j.issn.1004-7964.2024.03.003

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Microbial Physiological Effects and Product Transformation of Abiotic Humification

ZHU Chao¹, ZHOU Mingfang¹, LI Haonan¹, MA Hongrui^1,*, HAO Yongyong²

1. College of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;
2. College of Light Industry Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China

Received:2023-10-31 Revised:2023-12-13 Accepted:2023-12-15 Published:2024-06-01 Online:2024-04-25

Abstract

Abstract: The transformation behavior of dissolved organic matter in tannery wastewater is still unclear in biological treatment systems. Herein, tannic acid, glycine and glucose were used as raw materials to simulate the abiotic humification of wastewater. By combining with multivariate spectroscopy, we investigated the characteristics of the abiotic humus products, the transformation and the effects on the metabolic activity of microorganisms in both pure and mixed culture systems. Results showed that tannic acid promoted the abiotic humification process, while the fluorescence intensity of the amino acid-like region was enhanced at 48 h-1 000 mg/L in the pure culture after the microorganisms were exposed to the abiotic humification system. The fluorescence intensity was the strongest in the humic acid-like region at 96 h-2 000 mg/L in the mixed culture, and the fluorescence region of the solubilized microbial by-product-like products was added. Analysis of SUVA₂₅₄, a measure of the degree of humification, showed that the mixed culture was three times higher than the pure culture, indicating a significant increase in the aromaticity of the transformed abiotic humic products compared to the mixed culture. This work revealed that abiotic humification and its biotransformation products were important control factors governing the standardized treatment of tannery wastewater.

Key words: dissolved organic matter, abiotic humification, Escherichia coli, activated sludge

CLC Number:

ZHU Chao, ZHOU Mingfang, LI Haonan, MA Hongrui, HAO Yongyong. Microbial Physiological Effects and Product Transformation of Abiotic Humification[J]. Leather Science and Engineering, 2024, 34(3): 17-24.

References

[1] 王浩,雷超,曾运航,等.以制革废弃物制备的复合氨基酸材料及其清洁脱灰[J].皮革科学与工程,2022,32(6):13-18,31.
WANG H,LEI C,ZENG Y H,et al.Compound amino acids prepared by using leather waste for clean deliming[J].Leather Sci Eng,2022,32(6):13-18,31.(in Chinese)
[2] 刘杨,黄吉进,张泽天,等.单宁酸基荧光水性聚氨酯的制备与性能研究[J].皮革科学与工程,2022,32(6):39-44,51.
LIU Y,HUANG J J,ZHANG Z T,et al.Synthesis and characterization of tannic acid based fluorescent waterborne polyurethane[J].Leather Sci Eng,2022,32(6):39-44,51.(in Chinese)
[3] 栗玉鸿,李伟光,王勇,等.Fenton氧化法去除皮革废水中单宁的试验研究[J].水处理技术,2011,37(12):68-71.
LI Y H,LI W G,WANG Y,et al.Oxidation of tannins in tannery wastewater by fenton's reagent[J].Technol Water Treat,2011,37(12):68-71.(in Chinese)
[4] BARTOSZEK M,POLAK J,SUŁKOWSKI W W.NMR study of the humification process during sewage sludge treatment[J].Chemosphere,2008,73(9):1465-1470.
[5] KHEMAKHEM M,PAPADIMITRIOU V,SOTIROUDIS G,et al.Melanin and humic acid-like polymer complex from olive mill waste waters.Part I.Isolation and characterization[J].Food Chem,2016,203:540-547.
[6] 单超群,马宏瑞,朱超,等.有机分子聚合过程中DOM特征及其与蒙脱土的吸附过程[J].皮革科学与工程,2023,33(2):8-14.
SHAN C Q,MA H R,ZHU C,et al.Characteristics of DOM and its adsorption process with mont-morillonite during the polymerization of organic molecules[J].Leather Sci Eng,2023,33(2):8-14.(in Chinese)
[7] JOKIC A,ZIMPEL Z,HUANG P M,et al.Molecular shape analysis of a Maillard reaction intermediate[J].SAR QSAR Environ Res,2001,12(3):297-307.
[8] ZHU C,WANG H Q,MA H R,et al.Tanning process promotes abiotic humification:separation and characterization of humic acid-like polymers complex[J].Environ Sci Pollut Res Int,2020,27(33):41437-41445.
[9] 周姣. 制革废水生物处理中微生物代谢产物与铬的分布特征研究[D].桂林:桂林理工大学,2020.
ZHOU J.Characteristics of microbial metabolites and chromium distribution during tannery wastewater biological treatment[D].Guilin:Guilin University of Technology,2020.(in Chinese)
[10] LIU C,XIAO B,DAUTA A,et al.Effect of low power ultrasonic radiation on anaerobic biodegradability of sewage sludge[J].Bioresour Technol,2009,100(24):6217-6222.
[11] 王金翠,孙宝盛.胞外聚合物与溶解性微生物产物的关系[J].环境科学与技术,2008,31(11):18-20.
WANG J C,SUN B S.Relationship between extracellular polymeric substance and soluble microbial products[J].Environ Sci Technol,2008,31(11):18-20.(in Chinese)
[12] HAO Y Y,MA H R,WANG Q,et al.Refractory DOM in industrial wastewater:formation and selective oxidation of AOPs[J].Chem Eng J,2021,406:126857.
[13] 胡慧. 大肠杆菌在多介质中分布及迁移的试验研究[D].焦作:河南理工大学,2022.
HU H.Experimental study on the distribution and migration of Escherichia coli in multi-media[D].Jiaozuo:Henan Polytechnic University,2022.(in Chinese)
[14] 张蕊鑫,王智慧,丛威,等.一种用高效凝胶渗透色谱测试褐藻胶分子量及分子量分布的方法[J].过程工程学报,2020,20(1):99-107.
ZHANG R X,WANG Z H,CONG W,et al.Molecular weight and molecular weight distribution of alginate determination by gel permeation chromatography[J].Chin J Process Eng,2020,20(1):99-107.(in Chinese)
[15] ZHANG Y C,YUE D B,MA H.Darkening mechanism and kinetics of humification process in catechol-Maillard system[J].Chemosphere,2015,130:40-45.
[16] TAN K H.Humic matter in soil and the environment:principles and controversies[M].Boca Raton:CRC Press,2003.
[17] 王斌,黄廷林,李楠,等.水源水库沉积物及其上覆水DOM光谱特征[J].中国环境科学,2022,42(3):1309-1317.
WANG B,HUANG T L,LI N,et al.Spectral characteristics of dissolved organic matter in sediment and overlying water of water source reservoir[J].China Environ Sci,2022,42(3):1309-1317.(in Chinese)
[18] 孙颖,高颖,陈惠,等.亚热带米槠林不同更新方式对土壤可溶性有机质降解性的影响[J].应用生态学报,2020,31(4):1073-1082.
SUN Y,GAO Y,CHEN H,et al.Effects of different regeneration patterns on soil dissolved organic matter degradation in Castanopsis carlesii forests of subtropical China[J].Chin J Appl Ecol,2020,31(4):1073-1082.(in Chinese)
[19] 刘鑫慧,李华,轩滋,等.茶多酚对花生蛋白美拉德反应产物抗氧化性及加工特性的影响[J].河南工业大学学报(自然科学版),2023,44(1):36-43.
LIU X H,LI H,XUAN Z,et al.Effect of tea polyphenols on antioxidant and processing characteristics of maillard reaction products of peanut protein[J].J Henan Univ Technol(Nat Sci Ed),2023,44(1):36-43.(in Chinese)
[20] NEWCOMB C J.Humic matter in soil and the environment,principles and controversies[J].Soil Sci Soc Am J,2015,79(5):1520-1520.
[21] BERNHARDT J,WEIBEZAHN J,SCHARF C,et al.Bacillus subtilis during feast and famine:visualization of the overall regulation of protein synthesis during glucose starvation by proteome analysis[J].Genome Res,2003,13(2):224-237.
[22] 范春辉,张颖超,杜波,等.制革废水处理过程溶解性有机物的光谱特性研究[J].光谱学与光谱分析,2015,35(6):1587-1591.
FAN C H,ZHANG Y C,DU B,et al.Spectral analysis of dissolved organic matter of tannery wastewater in the treatment process[J].Spectrosc Spectral Anal,2015,35(6):1587-1591.(in Chinese)
[23] 朱永恒,白钰鹏.土壤腐殖质组型:土壤生物学和生态学研究的新视角[J].土壤通报,2021,52(6):1486-1495.
ZHU Y H,BAO Y P.Humus forms:a new perspective on soil biology and ecology research[J].Chin J Soil Sci,2021,52(6):1486-1495.(in Chinese)
[24] 何席伟,高洁,张徐祥,等.焦化废水生化处理过程中溶解性有机物及毒性变化规律[J].环境监控与预警,2022,14(2):15-24,31.
HE X W,GAO J,ZHANG X X,et al.Changes of dissolved organic matter and toxicity of coking wastewater during biochemical treatment[J].Environ Monit Forewarn,2022,14(2):15-24,31.(in Chinese)
[25] 郝晓地,周鹏,曹亚莉.污水处理中腐殖质的来源及其演变过程[J].环境工程学报,2017,11(1):1-11.
HAO X D,ZHOU P,CAO Y L.Origins and evolution processes of humic substances in wastewater treatment[J].Chin J Environ Eng,2017,11(1):1-11.(in Chinese)
[26] SIDDHARTH T,SRIDHAR P,VINILA V,et al.Environmental applications of microbial extracellular polymeric substance(EPS):a review[J].J Environ Manage,2021,287:112307.
[27] 傅平青. 水环境中的溶解有机质及其与金属离子的相互作用——荧光光谱学研究[D].贵阳:中国科学院研究生院(地球化学研究所),2004.
FU P Q.Dissolved organic matter in natural aquatic environments and its complexation with metal ions:a study based on fluorescence spectroscopy[D].Guiyang:Institute of Geochemistry,Chinese Academy of Science,2004.(in Chinese)

Microbial Physiological Effects and Product Transformation of Abiotic Humification

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