载四羧基酞菁锌明胶微球的制备及其光动力抗菌活性

doi:10.19677/j.issn.1004-7964.2023.06.004

皮革科学与工程 ›› 2023, Vol. 33 ›› Issue (6): 24-30.doi: 10.19677/j.issn.1004-7964.2023.06.004

载四羧基酞菁锌明胶微球的制备及其光动力抗菌活性

李梓童¹, 廖晓敏¹, 许诺¹, 徐洲², 陈意³, 钟琴^1,2,*, 常金明^1,3

1.西华师范大学化学化工学院,四川南充 637009;
2.宜宾学院固态发酵资源利用四川省重点实验室,四川宜宾 644000;
3.四川大学皮革化学与工程教育部重点实验室,四川成都 610065

收稿日期:2023-06-05 出版日期:2023-12-01 上线日期:2023-11-14
通讯作者: *钟琴,女,硕士研究生,从事抗菌功能材料研究。E-mail: zhongqin@cwnu.edu.cn。
作者简介:李梓童(2002-),男,从事生物质基抗菌材料研究。E-mail: lizitong1005@163.com。
基金资助:
固态发酵资源利用四川省重点实验室开放基金项目(2022GTYY05); 皮革化学与工程教育部重点实验室 (四川大学) 开放课题基金资助(2020)

Preparation and Photodynamic Antimicrobial Activity of Tetra(4-carboxyphenoxy)-Phthalocyaninatozinc(II) Loaded Gelatin Microspheres

LI Zitong¹, LIAO Xiaomin¹, XU Nuo¹, XU Zhou², CHEN Yi³, ZHONG Qin^1,2,*, CHANG Jinming^1,3

1. College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637000, China;
2. Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin 644000, China;
3. Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China

Received:2023-06-05 Online:2023-12-01 Published:2023-11-14

摘要/Abstract

摘要： 基于光敏剂的抗菌光动力疗法已成为抗生素治疗的一种替代治疗方法,但光敏剂水溶性差、易聚集的问题严重阻碍了抗菌光动力疗法的广泛应用。文章利用四羧基酞菁锌(TPZnPc)和明胶的羧基与Cu²⁺相互配位,采用油包水乳化交联法,制备载四羧基酞菁锌明胶微球(TPZnPc-GMs),使用傅里叶变换红外光谱、扫描电镜、紫外可见光谱等对微球进行了表征。结果表明:TPZnPc-GMs成功负载了TPZnPc,负载率为0.6%;微球平均粒径为1.6 μm,其中TPZnPc以单体形式存在于微球中。TPZnPc-GMs溶液在红光(630~710 nm)照射下能生成单线态氧,与革兰氏阳性枯草芽孢杆菌(B. subtilis)混合培养,当TPZnPc-GMs质量浓度为1 mg/mL时,光动力抑菌率为65%,较高质量浓度的TPZnPc-GMs(3 mg/mL)光动力抑菌率可达100%。

关键词: 明胶微球, 光动力抗菌, 光敏剂, 聚集

Abstract: Antimicrobial photodynamic therapy (APDT) based on photosensitizers has emerged as an alternative treatment to antibiotics. However, the poor water solubility and tendency to aggregate of photosensitizers severely impede the widespread application of APDT. Therefore, in this study, tetra(4-carboxyphenoxy)-phthalocyaninatozinc(II)-loaded gelatin microspheres (TPZnPc-GMs) were prepared by using a water-in-oil emulsion cross-linking method. The carboxyl groups in TPZnPc and gelatin were employed to coordinate with Cu²⁺ ions. The microspheres were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy and UV-visible spectroscopy. Results demonstrated the successful loading of TPZnPc into TPZnPc-GMs with a loading rate of 0.6%. The average particle size of TPZnPc-GMs was 1.6 μm, and TPZnPc remained its monomeric form within these microspheres. The TPZnPc-GMs solution, when exposed to red light (630-710 nm), produced singlet oxygen. When co-cultured with Gram-positive Bacillus subtilis (B. subtilis), the TPZnPc-GMs solution at a concentration of 1 mg/mL exhibited 65% photodynamic bactericidal rate. The photodynamic bactericidal rate of 100% was achieved when its concentration was improved to 3 mg/mL.

Key words: gelatin microspheres, photodynamic antimicrobial, photosensitizers, aggregation

中图分类号:

TQ469

李梓童, 廖晓敏, 许诺, 徐洲, 陈意, 钟琴, 常金明. 载四羧基酞菁锌明胶微球的制备及其光动力抗菌活性[J]. 皮革科学与工程, 2023, 33(6): 24-30.

LI Zitong, LIAO Xiaomin, XU Nuo, XU Zhou, CHEN Yi, ZHONG Qin, CHANG Jinming. Preparation and Photodynamic Antimicrobial Activity of Tetra(4-carboxyphenoxy)-Phthalocyaninatozinc(II) Loaded Gelatin Microspheres[J]. Leather Science and Engineering, 2023, 33(6): 24-30.

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载四羧基酞菁锌明胶微球的制备及其光动力抗菌活性

Preparation and Photodynamic Antimicrobial Activity of Tetra(4-carboxyphenoxy)-Phthalocyaninatozinc(II) Loaded Gelatin Microspheres

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