关键词: 生物絮凝剂, 克雷伯氏菌, 印染废水, 鉴定

Abstract: In order to solve the problems of secondary pollution of chemical flocculants, a strain EW-1 with flocculant-generating capacity was screened out from the biological treatment tank of wool mill wastewater, and identified as Klebsiella by morphological characteristics, physiological and biochemical analysis and 16S rRNA sequencing. The biological flocculant produced by the strain was purified and the chemical composition was determined. The results showed that the main components of the biological flocculant were polysaccharides, and the main groups included hydroxyl group, amide group, carboxyl group and carbonyl group. The single factor test of the bioflocculant used in the flocculation of printing and dyeing wastewater showed that the optimal dosage was 6 mL (flocculant concentration was 500 mg/L) and the optimal pH was 8-9.

Key words: biological flocculants, Klebsiella, printing and dyeing wastewater, identification