基于低温活性染料标记皮粉底物的蛋白酶性能表征方法

doi:10.19677/j.issn.1004-7964.2019.02.003

皮革科学与工程 ›› 2019, Vol. 29 ›› Issue (02): 18-.doi: 10.19677/j.issn.1004-7964.2019.02.003

基于低温活性染料标记皮粉底物的蛋白酶性能表征方法

制革清洁技术国家工程实验室(四川大学);皮革化学与工程教育部重点实验室(四川大学);四川大学轻纺与食品学院;

出版日期:2019-04-12 上线日期:2019-04-12
基金资助:
“十三五”国家重点研发计划项目(2017YFB0308402)

A Novel Method of Characterizing Activity of Protease towards Collagen based on Substrate of Hide Power Labeled with Low-temperature Reactive Dyestuff

1.National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China; 2.Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China; 3. College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China

Published:2019-04-12 Online:2019-04-12

摘要/Abstract

摘要： 建立了一种以低温活性染料标记的未变性皮粉(胶原纤维)为底物的快速测定蛋白酶活力的方法。优化了染色皮粉的制备方法,考察了底物的性质,优化了酶活力检测条件,并对几种蛋白酶的活力进行了测定。结果表明,染色后皮粉的结构未发生明显的改变,适合在较宽pH值范围进行蛋白酶活力测定,测定结果具有较好的重现性;蛋白酶不同底物作用性能有较大的差异,相对于酪蛋白和热变性染色皮粉,新建立的方法的测定结果对制革过程中酶的选用和使用条件的优化更具实际的指导意义。

关键词: 蛋白酶, 酶活力, 染色皮粉

Abstract: A method for rapid determination of protease activity based on the substrate of un-denatured hide powder (collagen fibers) labeled with a low temperature reactive dyestuff was developed. The preparation method of the substrate was optimized, the properties of substrate were investigated, the conditions of detection were optimized, and the activities of several proteases were measured. The results show that the structure of the dyed hide powder does not change significantly, and it is suitable for the determination of protease activity in a wide pH range. The performances of proteases towards different substrates are different. Compared with casein and heat-denaturated staining powder, the results of the new method have more practical guiding significance for the selection of proteases and the optimization of using conditions in the process of leather making.

Key words: protease, enzyme activity, Un-denatured dyeing substrate

中图分类号:

TS51

张茜, 田永鑫, 鲜娇, 张春晓, 李艳, 彭必雨, . 基于低温活性染料标记皮粉底物的蛋白酶性能表征方法[J]. 皮革科学与工程, 2019, 29(02): 18-.

ZHANG Xi, TIAN Yongxin, XIAN Jiao, ZHANG Chunxiao, LI Yanhong, PENG Biyu. A Novel Method of Characterizing Activity of Protease towards Collagen based on Substrate of Hide Power Labeled with Low-temperature Reactive Dyestuff[J]. Leather Science and Engineering, 2019, 29(02): 18-.

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基于低温活性染料标记皮粉底物的蛋白酶性能表征方法

A Novel Method of Characterizing Activity of Protease towards Collagen based on Substrate of Hide Power Labeled with Low-temperature Reactive Dyestuff

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