三种酸性蛋白酶等电点测试方法的比较

doi:10.19677/j.issn.1004-7964.2023.03.009

皮革科学与工程 ›› 2023, Vol. 33 ›› Issue (3): 50-56.doi: 10.19677/j.issn.1004-7964.2023.03.009

三种酸性蛋白酶等电点测试方法的比较

刘畅¹, 曾运航^1,2,*, 石碧^1,2

1.四川大学制革清洁技术国家工程实验室,四川成都 610065;
2.四川大学皮革化学与工程教育部重点实验室,四川成都 610065

收稿日期:2022-11-25 出版日期:2023-06-01 上线日期:2023-05-17
通讯作者: *曾运航（1985-）,女,教授,主要从事皮革清洁生产和制革用酶制剂研究。E-mail：zengyunhang@scu.edu.cn。
作者简介:刘畅（1998-）,女,硕士研究生。E-mail：526937617@qq.com。
基金资助:
国家自然科学基金（21878193）

Comparison of Three Test Methods for Isoelectric Point of Acid Protease

LIU Chang¹, ZENG Yunhang^1,2,*, SHI Bi^1,2

1. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China;
2. Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China

Received:2022-11-25 Online:2023-06-01 Published:2023-05-17

摘要/Abstract

摘要： 采用Zeta电位法、沉淀法和等电聚焦电泳（IEF）法测定了不同来源的四种酸性蛋白酶的等电点（pI）,并比较了这三种方法用于测量酸性蛋白酶pI的优缺点。结果表明：Zeta电位法适用于检测各种酸性蛋白酶,但需要指出的是,对于工业级酶制剂,该方法获得的pI值是其各组分pI值的综合结果;沉淀法难以观察到高溶解度蛋白酶的pI沉淀现象,但使用粒径分析仪检测酶溶液的粒径变化情况可以获得准确的pI;IEF法能准确分析pI大于3的酸性蛋白酶,但有些酸性蛋白酶制剂的酶蛋白组分的pI小于3,因此该方法不适用于检测所有的酸性蛋白酶。

关键词: 酸性蛋白酶, 等电点, Zeta电位, 沉淀法, 等电聚焦

Abstract: Isoelectric points (pIs) of four acid proteases with different sources were measured by using the methods of Zeta potential, precipitation and isoelectric focusing electrophoresis (IEF), respectively. The advantages and disadvantages of the three methods for measuring the pI of acid protease were compared based on the pI results. Results show that the Zeta potential method is suitable for testing various acid proteases. However, it should be mentioned that, for an industrial grade protease preparation, the pI value obtained by this method is a comprehensive value of its components’ pIs. For the precipitation method, it is difficult to observe the precipitation phenomena generated by a protease with high solubility at its pI. But an accurate pI value can be obtained by using a particle size analyzer to detect the change in particle size of protease solution. The IEF method can accurately test the pIs of acid proteases that are higher than 3. However, the pIs of protein components in some acid protease preparations are less than 3, therefore, this method is not suitable for testing all acid proteases.

Key words: acid protease, isoelectric point, Zeta potential, precipitation method, isoelectric focusing

中图分类号:

TS57

刘畅, 曾运航, 石碧. 三种酸性蛋白酶等电点测试方法的比较[J]. 皮革科学与工程, 2023, 33(3): 50-56.

LIU Chang, ZENG Yunhang, SHI Bi. Comparison of Three Test Methods for Isoelectric Point of Acid Protease[J]. Leather Science and Engineering, 2023, 33(3): 50-56.

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三种酸性蛋白酶等电点测试方法的比较

Comparison of Three Test Methods for Isoelectric Point of Acid Protease

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