三种酸性蛋白酶等电点测试方法的比较

doi:10.19677/j.issn.1004-7964.2023.03.009

Leather Science and Engineering ›› 2023, Vol. 33 ›› Issue (3): 50-56.doi: 10.19677/j.issn.1004-7964.2023.03.009

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Comparison of Three Test Methods for Isoelectric Point of Acid Protease

LIU Chang¹, ZENG Yunhang^1,2,*, SHI Bi^1,2

1. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China;
2. Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China

Received:2022-11-25 Published:2023-06-01 Online:2023-05-17

Abstract

Abstract: Isoelectric points (pIs) of four acid proteases with different sources were measured by using the methods of Zeta potential, precipitation and isoelectric focusing electrophoresis (IEF), respectively. The advantages and disadvantages of the three methods for measuring the pI of acid protease were compared based on the pI results. Results show that the Zeta potential method is suitable for testing various acid proteases. However, it should be mentioned that, for an industrial grade protease preparation, the pI value obtained by this method is a comprehensive value of its components’ pIs. For the precipitation method, it is difficult to observe the precipitation phenomena generated by a protease with high solubility at its pI. But an accurate pI value can be obtained by using a particle size analyzer to detect the change in particle size of protease solution. The IEF method can accurately test the pIs of acid proteases that are higher than 3. However, the pIs of protein components in some acid protease preparations are less than 3, therefore, this method is not suitable for testing all acid proteases.

Key words: acid protease, isoelectric point, Zeta potential, precipitation method, isoelectric focusing

CLC Number:

TS57

LIU Chang, ZENG Yunhang, SHI Bi. Comparison of Three Test Methods for Isoelectric Point of Acid Protease[J]. Leather Science and Engineering, 2023, 33(3): 50-56.

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Comparison of Three Test Methods for Isoelectric Point of Acid Protease

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