基于Cu(II)离子的复合抑制剂可降低酶脱毛过程中胶原纤维的水解

doi:10.19677/j.issn.1004-7964.2022.03.010

Leather Science and Engineering ›› 2022, Vol. 32 ›› Issue (3): 54-58.doi: 10.19677/j.issn.1004-7964.2022.03.010

Previous Articles Next Articles

Compounding Inhibitors based on Cu(II) Ions Reduce the Hydrolytic Efficiency of Collagen Fibers on Enzymatic Unhairing Process

LI He¹, ZHOU Xuewei¹, CHENG Haiming^1,2,*

1. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China;
2. Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China

Received:2022-01-20 Published:2022-06-28 Online:2022-05-16

Abstract

Abstract: In the enzymatic unhairing process, the risk of loosen and grain damage is mainly due to the excessive hydrolysis of collagen fibers by enzymes. In this experiment, compounding inhibitors were applied to reduce the excessive hydrolysis of collagen fibers during enzymatic unhairing process and to reduce the grain damage. The effect of compounding inhibitors on the enzymatic hydrolysis activity of AS1.398 were explored, and the compounding inhibitors based on Cu(II) ions were screened out and applied on bovine hide unhairing process. The results showed that the addition of 5 mmol/L of Cu(II) ions and 20 mmol/L of sodium succinate compounding inhibitor could maintain the enzymatic unhairing rate while inhibit the hydrolysis of collagen fibers. This study provides a potential novel strategy for industrial application of enzymatic unhairing process with safe and efficiency.

Key words: AS1.398 protease, enzymatic unhairing, compounding inhibitor, leather-making, Cu(II) ions

CLC Number:

TS541

LI He, ZHOU Xuewei, CHENG Haiming. Compounding Inhibitors based on Cu(II) Ions Reduce the Hydrolytic Efficiency of Collagen Fibers on Enzymatic Unhairing Process[J]. Leather Science and Engineering, 2022, 32(3): 54-58.

References

[1] 王学川,李飞虎,任龙芳,等.酶法脱毛技术的研究进展与展望[J].中国皮革,2012,41(13):49-52+55.
[2] Abdel-Mohsen S Ismail,Manal M.Housseiny,Heba I,et al.Novel keratinase from Trichoderma harzianum MH-20 exhibiting remarkable dehairing capabilities[J].International Biodeterioration&Biodegradation,2012,70:14-19.
[3] 陈梅,陈立炜,李鹤,等.蛋白酶K在黄牛皮酶脱毛中的应用[J].皮革科学与工程,2020,30(5):44-48.
[4] Wang R,Li ZQ,Chen M,et al.Isolation and purification of caseinase and collagenase from commolercial bacillus subtilis AS1.398 enzyme by affinity chromatography[J].Journal of the Society of Leather Technologists and Chemists,2009,93:8-11.
[5] 宋映,马远,曾运航,等.蛋白酶的相对分子质量对其在制革过程传质的影响[J].皮革科学与工程,2018,28(1):5-10+24.
[6] 宋金枝,王新德,曹珊,等.低胶原降解力蛋白酶用于制革脱毛工序的研究[J].中国皮革,2021,50(3):39-45.
[7] 高晓丽,刘毅,王睿,等.黄牛皮低温酶脱毛的研究[J].皮革科学与工程,2011,21(3):47-51+57.
[8] Chen M,Jiang MF,Li H,Cheng HM.Screening of additives to reduce grain damage risk on unhairing by proteinase K[J].Journal of Leather Science and Engineering,2020,2(1):9.
[9] Chen M,Jiang MF,Chen M,and Cheng HM.Approach towards safe and efficient enzymatic unhairing of bovine hides[J].Journal of the American Leather Chemists Association,2018,113(2):59-64.
[10] 江明芳. AS1.398蛋白酶中胶原酶活力的抑制及脱毛应用[D].成都:四川大学,2017.
[11] 许伟,郝丽芬.两种脱毛蛋白酶活力的研究[J].皮革化工,2006,(5):12-14+33.
[12] 西北轻工业学院.皮革分析检验[M].北京:中国轻工业出版社,1988:80-88.
[13] 江明芳,陈敏,程海明.胶原酶活性的测定方法[J].皮革科学与工程,2016,26(5):21-27.
[14] Lowry OH,Rosebrough NJ,Farr AL,et al.Protein measurement with the Folin phenol reagent[J].J Journal of biological chemistry,1951;193(1):265-275.
[15] Bergman I,Loxley R.Two Improved and Simplified Methods for the Spectrophotometric Determination of Hydroxyproline[J].Analytical Chemistry,1963,35(12):1961-1965.
[16] Dubois M,Gilles K A,Hamilton J K,Rebers PA,Smith F.A colorimetric method for the determination of sugars[J].Nature,1951,168(4265):167-167.
[17] 余茜,张国丽,敖晓琳.金属离子对微生物蛋白酶活性的影响及机理[J].中国食品学报,2019,19(4):287-294.
[18] 高磊. 基质金属蛋白酶活性中心肽段的合成,纯化及与金属离子作用研究[D].聊城:聊城大学,2009.
[19] Li K,Tay F R,Yiu C K Y.The past,present and future perspectives of matrix metalloproteinase inhibitors[J].Pharmacology&therapeutics,2020,207:107465.

Compounding Inhibitors based on Cu(II) Ions Reduce the Hydrolytic Efficiency of Collagen Fibers on Enzymatic Unhairing Process

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	LIU Xianjun, SHAN Zhihua. Action Characteristics of Suspending Revolving Drum in Modern Tanning Process [J]. Leather Science and Engineering, 2024, 34(1): 47-52.
[2]	TIAN Yongxin, GAO Mengchu, ZHANG Xu, PENG Biyu, ZHANG Chunxiao, CHEN Yukun. Effect of Calcium Salt Mineralization on Protease Resistance of Hide Collagen Fibers and Enzymatic Unhairing [J]. Leather Science and Engineering, 2021, 31(3): 1-6.
[3]	JIANG Mingfang, CHENG Haiming. Visual Metrological Analysis of Leather-Making Research Literature Based on CNKI（2000-2019） [J]. Leather Science and Engineering, 2020, 30(5): 78-83.
[4]	CHEN Mei, CHEN Liwei, LI He, CHEN Min, CHENG Haiming. Application of Protease K on Enzymatic Unhairing of Bovine Hide [J]. Leather Science and Engineering, 2020, 30(5): 44-48.