胶原静电纺丝用水性溶剂体系对鱼胶原结构的影响

doi:10.19677/j.issn.1004-7964.2019.06.001

Leather Science and Engineering ›› 2019, Vol. 29 ›› Issue (06): 5-10.doi: 10.19677/j.issn.1004-7964.2019.06.001

Effects of Water-based Solvents used for Collagen Electrospinning on the Structure of Fish Collagen

LIU Wentao^1,2*, GONG Like¹, GUO Zhengguo¹

1. Key Laboratory of Leather Chemistry and Engineering Sichuan University, Ministry of Education, Chengdu 610065, China;
2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China

Received:2019-09-15 Published:2019-12-28 Online:2020-03-27

Abstract

Abstract: The effects of two kinds of water-based solvents used for collagen electrospinning (acetic acid solution and PBS/ethanol mixed solvent) on the structure of snakehead skin collagen were studied. Firstly, the stability and surface tension of the water-based solvents reported in literatures were analyzed. On this basis, three concentrations (0.5 mol/L, 1 mol/L, and 2 mol/L) of acetic acid solution and three types of PBS/ethanol mixed solvent (V(PBS)∶V(ethanol) equals to 3∶1, 3∶0.5, and 3∶0.25) were used to prepare fish collagen solutions. The techniques including electrophoresis, infrared and ultraviolet spectroscopy and ultra-sensitive differential scanning calorimetry were used to study the structural properties of different fish skin collagen solutions. Results showed that the two kinds of water-based solvents destroyed the structural stability of fish collagen with the increase of acetic acid or ethanol concentration, and the denaturation effect of ethanol on fish collagen was stronger. Acetic acid solution maybe more suitable for continuous stable electrospinning of fish collagen than PBS/ethanol mixed solvent, but it is necessary to take a strategy to simultaneously enhance the structural stability and electrostatic spinnability of collagen.

Key words: fish collagen, electrospinning, water-based solvents, structural stability, denaturation

CLC Number:

TS512

LIU Wentao, GONG Like, GUO Zhengguo. Effects of Water-based Solvents used for Collagen Electrospinning on the Structure of Fish Collagen[J]. Leather Science and Engineering, 2019, 29(06): 5-10.

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Effects of Water-based Solvents used for Collagen Electrospinning on the Structure of Fish Collagen

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