Abstract: Collagen hydrogel of bovine achilles tendon can be widely used in biomedical materials and other fields, but its physical and chemical properties need to be improved. In this study, three typical polyphenols, proanthocyanidins(PA), gallic acid, and epigallocatechin gallate, were selected to crosslink collagen hydrogels, and the water holding capacity, swelling rates, mechanical properties and rheological properties of the obtained cross-linked hydrogels as well as the micro-morphology of assembled collagen molecules were measured. The results showed that the water holding capacity of collagen hydrogel decreased after being crosslinked by three polyphenols, but all of them were above 95%. With the increase of polyphenol concentration, the swelling rate of hydrogel decreased, and the mechanical properties, enzymatic hydrolysis resistance and dynamic rheological properties were gradually enhanced, among which the physicochemical properties of collagen hydrogel crosslinked by 9 mmol/L PA were significantly improved. The hardness reached to 882 g; the degradation rate was only 2.47%; the energy storage modulus was greater than 4 000 Pa. This work is expected to provide certain theoretical and technical support for the high-value utilization of bovine achilles tendon.