Abstract: Two deep eutectic solvents (DES), sodium acetate trihydrate (SAT)-urea and choline chloride (ChCl)-oxalic acid dihydrate (H₂C₂O₄·2H₂O), were synthesized assolventsto dissolve gelatin.The differenceinstructure and property of gelatin before and after regeneration was investigated. The result indicated that the optimum stoichiometric ratios of SAT-urea DES and ChCl-H₂C₂O₄·2H₂O DES weren(SAT)/n(urea)=0.67 and n(ChCl)/n(H₂C₂O₄·2H₂O)=1.00, respectively. The chemical molecular structure of gelatin was not changed by DES, but the hydrogen bondsamong gelatin molecules were destroyed to some extent, leading to slightly lower crystallinity and thermal stability.SAT-urea DES had a stronger ability to form hydrogen bonds with gelatin and a higher degree to break the intermolecular hydrogen bonds of gelatin, but the damage degree of the tri-helix conformation was relatively low, so it was more suitable for dissolving gelatin.