Abstract: A class of nonionic surfactant (F4-PEG600-F4) containing double short fluorocarbon chains was one-pot synthesized using polyethylene glycol-600, isophorone diisocyanate and 1H,1H,2H,2H-perfluorohexan-1-ol as raw materials. By Karl Fischer method, the water content was measured to be below 0.1% (active ingredients > 99.9%). The structure of F4-PEG600-F4 was characterized by FT-IR and 1H-NMR, and its surface activity was studied in aqueous solution. The surface tension results show that the critical micelle concentration of F4-PEG600-F4 was 0.40 mmol/L, and the surface tension could be reduced to 21.0 mN/m (25 ℃). Moreover, when 1.0% NaCl was added or the pH was adjusted to be 3.0-10.0, the critical surface tension of F4-PEG600-F4 remained basically to be about 21.0 mN/m, showing its good electrolyte and pH resistance stability. Meanwhile, F4-PEG600-F4 was used as a fatliquoring agent in the fatliquoring process of chrome-tanned goatskin. The research results showed that under the conditions of 1% dosage and fatliquoring temperature of 40 ℃, the resulting leather exhibited the best overall performance, and its softness and mechanical properties were significantly improved. Its tensile strength, elongation at break and tear strength were increased by 19.0%, 4.2% and 18.5% respectively, and the waterproof performance of the leather surface was also significantly enhanced, too.