Abstract: One method for determining the total amount of persistent pollutants perfluorooctane sulfonic acid, its potassium salts (PFOS) and perfluorooctane sulfonamide (PFOSA) in leather was established by using gas chromatography-mass spectrometry. PFOS can not be directly detected. It can react with tetrabutyl ammonium hydroxide to generate corresponding ammonium salt, which was pyrolyzed into perfluorooctane sulfonate butyl ester at the position of the injection port of the instrument. Then the perfluorooctane sulfonate butyl ester and PFOSA can be simultaneously detected. The optimal sample pretreatment method, sample salification and derivatization conditions, and instrument working parameter conditions were optimized. The detection limit of this method is 0.14 mg/kg. The average recovery rate of is 89.52%- 99.16%, and the repeatability is good.