Abstract: An ultra high performance liquid chromatography tandem-mass spectrometry (UPLC-MS/MS) method was developed for the identification of animal origins for leather based on the peptide markers of porcine, bovine and sheep skins. Proteins were firstly extracted from the leather samples under high temperature and high pressure, and then digested by enzymolysis. The peptide solutions were separated by C₁₈ column and eluted with the aqueous solution of formic acid and acetonitrile as mobile phases. The animal origins of the leather samples were identified by the peaks of peptide markers in the peptide solution by multiple reaction monitoring (MRM) scanning. The established method has strong specificity, wide application range, good accurateness and reliablity, and provides a new technical means for leather identification.