Abstract: A method for determining the content of p-phenylenediamine, o-phenylenediamine and m-phenylenediamine in footwear leather components was established by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. Ultrasonic treatment was carried out for two times using methanol as solvent. Then the purified extracting solution was analyzed by HPLC-MS/MS and quantified by external standard method. The results showed that the linearly dependent coefficients of 3 kinds of phenylenediamines are all above 0.9997 in the concentration range of 5-500 μg/L, demonstrating good linear relationships. According to the condition of this study, the detection limits of 3 kinds of phenylenediamines are 0.02-0.03 mg/kg, and the average recovery ratios ranged from 89% to 106%, while the relative standard deviations of 3%-9% were obtained. The developed method was applicable for the determination of p-phenylenediamine, o-phenylenediamine and m-phenylenediamine residues in footwear leather components.