Abstract: Chrome tanning is currently the most mature, reliable, and suitable tanning method in leather industry. However, since the leather production needs to add a variety of chemicals, the discharge of the waste solid, liquid, and gas can pollute the environment. Therefore, pollution has become the biggest constraint for the development of leather industry. The leather production process involves multiple stages, multiple materials, and complex operations. Scientific and reasonable pollution evaluation of leather production has become a key issue related to the development of the leather industry. Life cycle assessment (LCA) is an assessment method that can quantitatively evaluate the environmental impact of a product’s life cycle from raw hide to leather. Based on the principles and theoretical framework of LCA, this paper adopted the eFootprint supply LCA network platform, global warming potential (GWP), primary energy consumption (PED), acidification potential (AP), etc. as environmental impact assessment indicators. Also, the main processes of leather production were evaluated throughout the LCA. This work provided a reference example for the establishment of the LCA database for leather industry, and gave the information support for the realization of the green industrial chain of the leather industry in China.