Abstract: The insoles were made from Lihu antimicrobial lining leather. The effect of the odor of human feet was evaluated through fitting test and the bacteria inhibitive rate of each test stage was detected byoscillatory method. The results showed that during fitting test, more than 75% of the subjects showed a decrease in odor level and duration of odor after trying on them. Bacterial inhibitive rate of staphylococcus aureus candida albicans and red trichopyton rubrum were 99.99%, 99.99% and 99.99% before 30 days of test, 82.65%, 85.76% and 88.59% for the antibacterial insoles after 30 days of test. As a result, this kind of antibacterial insole leather has a good inhibitory effect on bacteria and fungi. It can effectively suppress the odor and improve the environment of shoe, and the effect is remarkable.