Abstract: The thermal stability of aquatic collagen isolated from various sources and the applications of aquatic collagen in biomedical field were summarized in order to provide useful reference for the selection and application of aquatic collagen. In the thermal stability part, the effect and difference of various fish species, growth environment and tissue location on the thermal denaturation temperature of collagen were reviewed. In the biomedical application part, the focus was played on wound dressing, biomimetic bone materials, drug loading and sustained-release materials.